52个甘蔗品种在广西受病毒侵染情况

Virus Infection Situation of Fifty-two Sugarcane Varieties in Guangxi

为明确国家糖料体系甘蔗集成示范及区试品种在广西被病毒侵染情况,2017年从广西北海、南宁、崇左、百色、来宾、柳城、桂林等地集成示范及区试的52个甘蔗品种上采集带有甘蔗花叶病、甘蔗黄叶病和甘蔗杆状病毒病显著病症或不显病症叶片样品,采用特异引物通过RT-PCR和PCR方法进行5种病毒检测(甘蔗黄叶病毒、甘蔗线条花叶病毒、高粱花叶病毒、甘蔗花叶病毒和甘蔗杆状病毒)。结果表明,SCYLV的平均检出率为25.00%;SCSMV的平均检出率为7.97%;SrMV的平均检出率为7.69%;SCMV的平均检出率最低,为7.42%;SCBV的平均检出率最高,为68.41%,远远高于其他病毒。7个地方的甘蔗受病毒混合侵染现象普遍存在,北海的病毒混合侵染率甚至高于单一病毒侵染率。52个甘蔗品种在广西受5种病毒的总侵染率为79.67%,对甘蔗的生产安全存在着严重的潜在威胁,建议推广种植甘蔗脱毒种苗来缓解甘蔗病毒病害的危害。

To understand the virus infection situation of the integrated demonstration and regional test sugarcane varieties in the national sugar system in Guangxi,leaf samples with or without symptoms of sugarcane mosaic disease,sugarcane yellow leaf disease and sugarcane bacilliform disease were collected from 52 sugarcane varieties in 7 sugarcane areas in Guangxi,including Beihai,Nanning,Chongzuo,Baise,Laibin,Liucheng,Guilin.Five kinds of viruses were detected by RT-PCR and PCR with specific primers,including Sugarcane yellow leaf virus(SCYLV),Sugarcane streak mosaic virus(SCSMV),Sorghum mosaic virus(SrMV),Sugarcane mosaic virus(SCMV)and Sugarcane bacilliform virus(SCBV).The average detection rate of SCYLV,SCSMV,SrMV,SCMV,SCBV was 25%,7.97%,7.69%,7.42%and 68.41%,respectively.Mixed infection of different viruses was widespread in the seven sugarcane areas,the mixed infection rate in Beihai was even higher than the single virus infection rate.The total infection rate of 52 sugarcane varieties affected by the five viruses was 79.67%in Guangxi.There are serious potential threats to the safety of sugarcane production.It is recommended to plant virus-free seedlings to control the occurrence of sugarcane virus diseases.