苯甲酸与赤霉素对中国水仙切花保鲜效果的影响

Effect of Benzoic Acid and Gibberellin on Preservation of Cut Chinese Narcissus Flowers

本研究以中国水仙‘金盏银台’切花为材料,在基础瓶插液(1%蔗糖)中分别添加不同浓度苯甲酸与赤霉素,通过观测切花的瓶插寿命筛选适宜的保鲜剂配方;同时测定了这一过程中切花的鲜重变化,丙二醛(malondialdehyde,MDA)、游离脯氨酸(proline,PRO)和可溶性蛋白质的含量,超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)和过氧化氢酶(catalase,CAT)活性,分析保鲜剂发挥作用的生理生化基础。结果表明,1%蔗糖溶液添加200 mg/L苯甲酸的复合瓶插液可延长中国水仙切花瓶插寿命1.6 d;瓶插期间MDA含量呈上升趋势;瓶插过程中SOD和CAT活性、可溶性蛋白含量等高于同期对照组。1%蔗糖溶液添加200 mg/L赤霉素可延长切花瓶插寿命2.5 d;瓶插过程中SOD和POD活性、可溶性蛋白含量等高于同期对照组;但PRO含量在第15天较对照下降了40%。综合来看,1%蔗糖溶液添加200 mg/L赤霉素对中国水仙切花的保鲜效果最好。

In this study,different concentrations of benzoic acid and gibberellin(GA)were added to the basic vase cuttings(1%sucrose)of Chinese narcissus cut flowers.The suitable preservatives were screened by observing the vase life of cut flowers.The fresh weight changes,malondialdehyde,proline and soluble protein contents,superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities were also determined.The physiological and biochemical basis of the effect of preservatives was analyzed.The results showed that 1%sucrose solution with 200 mg/L benzoic acid could prolong the vase life of the cut flowers for 1.6 days,the content of malondialdehyde increased during the vase period,the activity of SOD and CAT,and the content of soluble protein in the vase process were higher than those in the control group during the same period.The vase life of the cut flowers was prolonged by adding 200 mg/L gibberellin in 1%sucrose solution for 2.5 days.SOD and POD activities and soluble protein content in vase were higher than those in the control group at the same time,but proline content decreased by 40%on the 15th day.In general,the effect of adding 200 mg/L gibberellin to 1%sucrose solution on preservation of Chinese narcissus cut flowers is the best.