肝细胞生长因子对高糖诱导的腹膜纤维化的影响

Effect of hepatocyte growth factor on peritoneal fibrosis induced by high glucose

目的探讨外源性肝细胞生长因子(Hepatocyte Growth Factor,HGF)对腹膜纤维化的影响。方法将HPMCs通过胰蛋白酶消化法从大网膜组织中分离并实施原代培养。后取第三代HPMCs,并在不同葡萄糖浓度(5.5、30、60 mmol/L D-葡萄糖)环境下持续培养48 h后对FN蛋白、TGF-β1水平进行检测、比较,并在60 mmol/L葡萄糖溶液中加入不同浓度的rhHGF(25、50、100 ng/mL)检测FN蛋白、TGF-β1水平,检测方式以ELISA方式开展。结果(1)HPMCs在高糖环境下TGF-β1、FN蛋白水平均较N组明显增加(P<0.05),呈现剂量依赖关系(P<0.05);(2)外源性HGF可对HPMCs在TGF-β1、FN的表达产生抑制效果(P<0.05),呈现剂量依赖关系(P<0.05)。结论高糖环境有利于促进HPMCs的TGF-β1表达,同时促进HPMCs合成ECM;高糖诱导ECM过程可在外源性HGF影响下发生抑制,提示在腹膜纤维化过程中HGF具有抑制作用,为PD相关腹膜纤维化的预防及治疗提供理论依据。

Objective To explore the effect of exogenous hepatocyte growth factor(HGF)on peritoneal fibrosis.Methods HPMCs were isolated from the greater omentum issue by tryps in digestion,and primary culture was carried out.After that,the third-generation HPMCs were and cultured continuously for 48 hours at different glucose concentrations(5.5,30,60 mmol/L D-glucose)and FN protein and TGF-β1 levels were detected,in the 60 mmol/L glucose environment added with rhHGF of different concentrations(25,50,100 ng/mL)and FN protein and TGF-β1 levels were detected and compared by ELISA.Results(1)TGF-β1 and FN protein levels of HPMCs in the high glucose environment were significantly increased compared with those in the N group(P<0.05)and a dose-effect relationship was observed(P<0.05).(2)The expression of TGF-β1 and FN in HPMCs was inhibited by exogenous HGF(P<0.05),and a dose-effect relationship was observed(P<0.05).Conclusion High glucose environment promotes the expression of TGF-β1 and the synthesis of ECM in HPMCs.The process of high glucose inducing ECM can be inhibited under the influence of exogenous HGF,suggesting that HGF has an inhibitory effect in the process of peritoneal fibrosis,which provides theoretical basis for the prevention and treatment of PD related peritoneal fibrosis.