摘要
目的:研究β1,3-N-乙酰葡萄糖胺转移酶8(β3Gn-T8)对胃癌细胞及胃癌移植瘤生物学行为的影响。方法:将β3Gn-T8正义表达载体pEGFP-C1-β3Gn-T8转染人胃腺癌AGS细胞,通过MTT法和活细胞计数检测细胞的增殖能力,流式细胞术检测细胞凋亡率。建立AGS细胞皮下移植瘤模型,检测移植瘤的生长情况。结果:转染pEGFP-C1-β3Gn-T8的AGS细胞活力明显增加(P<0.05),但是凋亡率无明显变化。转染pEGFP-C1-β3Gn-T8的移植瘤生长速度及最终体积均较对照组明显增加(P<0.05)。结论:β3Gn-T8可以促进胃癌AGS细胞的活力,明显促进AGS细胞移植瘤的生长。
AIM: To study the effects of β1,3-N-acetylglucosaminyltransferase 8(β3 Gn-T8) on the biological behaviors of gastric cancer cells and xenograft tumors. METHODS: After pEGFP-C1-β3 Gn-T8 was transfected into human gastric adenocarcinoma AGS cells, the cell proliferation was measured by MTT assay and viable cell counting with trypan blue exclusion, and the apoptotic rate was analyzed by flow cytometry. The model of AGS cell subcutaneous xenograft was established to detect the growth of the xenograft. RESULTS: The proliferation of AGS cells transfected with pEGFP-C1-β3 Gn-T8 was significantly increased(P<0.05), but the apoptotic rate was not significantly changed. The growth rate and final volume of xenograft tumor in pEGFP-C1-β3 Gn-T8 group were significantly higher than those in control group(P<0.05). CONCLUSION: β3 Gn-T8 promotes the proliferation of gastric cancer AGS cells and significantly increases the growth of AGS cell xenograft tumor.
作者
刘振华
沈力
吴士良
LIU Zhen-hua;SHEN Li;WU Shi-liang(Department of Pathology,Suzhou Health Vocational College,Suzhou 215009,China;Department of Biochemistry and Molecular Biology,Medical College of Soochow University,Suzhou 215123,China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2020年第1期152-156,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30670462
No.31170772)
