基于不同激素组合的紫丁香成熟种子组培诱导研究

Study on Tissue Culture Induction of Syringa oblata Mature Seeds Base on Different Hormone Treatments

以紫丁香成熟种子为外植体,采用不同激素组合培养基进行组培诱导试验的结果表明:采用中间切割方式的种子萌发率较高,最高可达59.72%;NAA与BA组合诱导丁香种子效果较好,NAA0.1 mg/L+BA1.0 mg/L的诱导率可达88.89%,MS+BA3.0 mg/L为顶芽和带芽茎段的最适增殖继代培养基;茎芽在试管内、外生根时间分别为20天和4-6周,生根率均为100%,紫丁香成熟种子组织培养试验取得成功。

Using Syringa oblata mature seeds as explants, different hormone combination media were used for tissue culture induction experiments.The results showed that the germination rate of seeds was highest with intermediate cutting method(up to 59.72%). The highest induction rate was 88.89% with the combination of 0.1 mg/L NAA and 1.0 mg/L BA. The optimum culture medium for terminal bud and stem with buds was MS + BA 3.0 mg/L. The rooting time of shoot was 20 days in vitro and 4-6 weeks ex vitro,respectively, with 100% of the rooting rate. Our results suggested that the tissue culture of S. oblata was successful using mature seed as explants.