高粱酒糟糖化处理及其暗发酵产氢性能

Saccharification of sorghum distiller′s grain and its performance for hydrogen production via dark fermentation

高粱酒糟是白酒生产过程中产生的富含纤维素的副产品,其暗发酵产氢被认为是较有前景的处理方法之一。为提高高粱酒糟氢气转化效率,探讨了不同预处理方式对高粱酒糟糖化率及糖化液发酵产氢的影响。结果表明,纤维素酶解是酒糟糖化的最优处理方式,其酶解条件为:固液比1:15(g:mL)、纤维素酶添加量4000 U/g,对应酶解液还原糖产率为17.21%,比对照组提高了341.28%。此外,糖化液产氢结果表明,与纤维素酶单一酶解方法相比,纤维素酶-淀粉酶耦合法的酒糟糖化液产氢率更高,对应的氢气产率为51.56 mL/g。扫描电镜结果显示,酒糟的纤维结构在酶解过程中明显被破坏,说明纤维素酶解促进了纤维素向糖类物质转化。

Sorghum distiller's grain is a cellulosic by-product from Chinese liquor fermentation.Dark fermentation is considered to be one of the most promising approaches for treating sorghum distiller's grain,along with biohydrogen production.To improve the hydrogen yield from sorghum distiller's grain,saccharification of sorghum distiller's grain and the hydrogen production rate were investigated.Results showed that the optimum approach for sorghum distiller's grain saccharification was to use enzymatic method,and the corresponding solid/liquid ratio and cellulase amount were 1∶15 and 4000 U/g,respectively.The obtained sugar yield reached 17.21%,which was 341.3% higher than that of the control group.Results of dark fermentation illustrated that a higher hydrogen yield(51.56 mL/g)was obtained with the addition of cellulase and amylase.Results of the scanning electron microscope showed that the surface structure of distiller's grains was significantly destroyed by the additional cellulase,indicating the conversion of cellulose to sugar was achieved.