Study on Differentiation of Growing Points in Corn Sprouts into Multiple Stems

[Objectives] This study was conducted to improve the efficiency of genetic transformation using growing points of corn sprouts. [Methods] A mixed liquid of plant hormones 6-BA and KT at a suitable concentration and ratio was added dropwise to the growing points of corn sprouts, followed by culture under appropriate conditions. [Results] Corn sprouts could be induced to form multiple plants, thereby improving the transformation efficiency of growing points in corn sprouts. [Conclusions] This study provides a breeding method using growing points of corn sprouts as transformation receptors for direct plantlet formation without tissue culture by the Agrobacterium transgenic technology.