结肠镜活检组织DKK3基因甲基化检测及其临床价值

Detection of DKK3 Gene Methylation in Colonoscopy Biopsy Tissue and Its Clinical Value

目的:检测结肠癌患者结肠镜活检组织DKK3基因(DKK3)甲基化并研究其临床意义。方法:选择结肠癌患者75例(COC组)和正常人75例(CON组)为研究对象,检测两组结肠镜活检组织DKK3甲基化率,分析COC组结肠镜活检组织不同临床病理中DKK3甲基化的差异,比较COC组结肠癌患者中结肠镜活检组织DKK3甲基化与未甲基化R0切除率、5年生存率及总生存时间的差异。结果:COC组结肠癌患者结肠镜活检组织DKK3甲基化率显著高于CON组正常人(P<0. 05)。COC组DKK3甲基化率在分化程度、肿瘤最大径、淋巴结转移、远处转移、Dukes分期和手术切除方面有差异(均P<0. 05)。姑息性手术、C+D期、肿瘤最大径≥5cm、低+中分化、有远处转移、有淋巴结转移患者DKK3甲基化率高于根治性手术、A+B期、肿瘤最大径<5cm、高分化、无远处转移及无淋巴结转移患者(均P<0. 05)。DKK3甲基化患者R0切除率、5年生存率及总生存时间均显著低于未甲基化患者(均P<0. 05)。结论:结肠癌患者结肠镜活检组织DKK3甲基化显著升高,与病情及预后紧密相关,可作为结肠癌病情及预后评估的标志物。

Objective: To detect the methylation of DKK3 gene in colonoscopy biopsy tissues of colon cancer patients and study its clinical significance. Methods: Choose colon cancer patients group( COC) and normal group( CON) as the research object,detection of COC and CON group colonoscopy biopsy tissue DKK3 methylation,analysis the COC group colonoscopy biopsy samples of different DKK3 methylation differences in clinical pathology,the COC groups colonoscopy in patients with colon cancer biopsy samples DKK3 methylation and methylation R0 resection rate,the differences between the 5-year survival rate and total survival time. Results: The methylation rate of DKK3 in colonoscopy biopsy tissues of colon cancer patients in COC group was significantly higher than that in CON group( P<0. 05). In the COC group,there were differences in the methylation rate of DKK3 in differentiation degree,maximum diameter of tumor,lymph node metastasis,distant metastasis,Dukes staging and surgical resection( all P<0. 05). DKK3 methylation rate in patients with palliative surgery,stage C + D,tumor maximum diameter ≥5 cm,low + medium differentiation,distant metastasis and lymph node metastasis was higher than that in patients with radical surgery,stage A + B,tumor maximum diameter<5 cm,high differentiation,no distant metastasis and no lymph node metastasis( P<0. 05). The R0 resection rate,5-year survival rate and total survival time of patients with DKK3 methylation were significantly lower than those without methylation( P<0. 05). Conclusion: DKK3 methylation in colonoscopy biopsy tissues of colon cancer patients is significantly increased,which is closely related to the disease and prognosis,and can be used as a marker for the evaluation of the disease and prognosis of colon cancer.