HPLC法比较不同产地枇杷叶中倍半萜苷A含量的差异

Comparison on difference in sesquiterpene glycoside A content in Eriobotrya japonica leaves from different localities by HPLC method

枇杷叶为蔷薇科(Rosaceae)植物枇杷[Eriobotrya japonica(Thunb.)Lindl.]的干燥叶,为中国常用的传统中药材,味苦,性微寒,具有清肺止咳、降逆止呕、抗氧化、抗炎、降血糖和保肝护肝的功效[1-3]。枇杷叶的主要成分包括黄酮类[4]、三萜酸类[5-7]、倍半萜苷类[8]、多酚[9]和多糖[10]等。现代药理学研究结果表明:枇杷叶中倍半萜苷类成分可缓解高脂饮食诱导的非酒精性脂肪肝[11],枇杷叶中的倍半萜苷A具有显著的降血糖作用[12]。

Taking Eriobotrya japonica(Thunb.) Lindl. leaves from 12 localities in 10 provinces(autonomous regions) as research materials, HPLC method for determination of sesquiterpene glycoside A content in E. japonica leaves was established, and sesquiterpene glycoside A contents in E. japonica leaves from different localities were determined and compared. The results show that sesquiterpene glycoside A contents in E. japonica leaves from different localities are 0.613-1.412 mg·g-1, and there are obvious differences among different localities. In which, sesquiterpene glycoside A content in E. japonica leaves from Wuzhou of Guangxi locality is the highest, that in E. japonica leaves from Kaifeng of He’nan locality is the lowest, and there is a significant difference between these two localities. It is suggested that Wuzhou of Guangxi is superior locality of E. japonica leaves.