儿茶酚胺浓度及β1—肾上腺素受体基因多态性与最大有氧能力的关联性

Association between the catecholamine concentration,Polymorphism of β1 Adrenergic Receptor Gene and Maximum Aerobic Capacity

目的:研究儿茶酚胺浓度及β1-肾上腺素受体基因(β1AR)多态性与运动能力的关联性。方法:160名体育专业学生为实验组,160名非体育专业学生为对照组,进行递增定量负荷运动后,测定血液儿茶酚胺浓度,并以PCR-RFLP检测β1AR49、389位点多态性。结果:运动后,儿茶酚胺浓度实验组高于对照组(P<0.05);肾上腺素浓度β1AR389位点CC基因型实验组明显高于对照组(P<0.01),49位点的AG基因型高于AA型(P<0.01),389位点的CC基因型明显高于CG型(P<0.01);去甲肾上腺素浓度方面,389位点CC实验组明显高于对照组(P<0.01),49位点的AG基因型明显高于AA型(P<0.01);多巴胺浓度方面,49位点的AG高于AA型(P<0.01)。有氧代谢能力指标VO2max/kg、VCO2max、RQmax、MVV、RFmax、Tmax、O2Plusemax、Tmax、Wmax-T、运动后即刻和运动后3min心率等指标中,实验组的β1AR基因389位点的CC明显高于对照组,并且实验组的CC高于CG和GG组(P<0.05/0.01),而恢复后心率所有实验组均低于对照组,差异均具有显著统计学意义(P<0.05)。结论:运动后,运动员的血液儿茶酚胺浓度升高显著;具有β1AR的389位点C等位基因者,运动能力较好,具有G等位基因者,运动能力较差。

Objective:To study the association between the catecholamine concentration,adrenergic receptor gene beta 1(β1AR) polymorphism and the exercise ability.Methods:160 physical education students were as the experimental group and 160 non-physical education majors as the control group.After the incremental quantitative load exercise,the concentration of catecholamine in the blood was measured and the polymorphism of the β1AR 49 and 389 loci was detected by the PCR RFLP.Results:The concentrations of three catecholamine in the experimental group were higher than those in the control group.The concentration of adrenaline in the experimental group was higher than that in the control group at CC the 389 loci(P<0.01) while it of AG genotype was higher than that of AA genotype at 49 loci(P<0.01)That of CC was significantly higher than that of CG at 389 loci(P<0.01).The concentration of norepinephrine in the experimental group was higher than that in the control group at CC 389 loci(P<0.01) while it of AG genotype was higher than that of AA genotype at 49 loci(P<0.01),The concentration of norepinephrine in the experimental group was higher than that in the control group at CC 389 loci(P < 0.01) while it of AG genotype was significantly higher than that of AA genotype at 49 loci(P<0.01).The concentration of dopamine in AG at 49 locus was significantly higher than that of AA(P<0.01).In terms of VO2 max/kg,VCO2 max,RQmax,MVV,RFmax,Tmax,O2 Plusemax,Tmax,Wmax-T,heart rate immediately and 3 min after exercise of aerobic capacity indicators,those in the experimental group was significantly higher than those in the control group in CC at t 389 loci,and those in CC was significantly higher than those in CG,GG(P<0.05/0.01),but heart rate after recovery in experimental groups was lower than that in the control group(P<0.05).Conclusion:the concentration of catecholamine increased significantly after exercising;those with C allele at the 399 in β1AR had better exercise ability,those with G allele had worse exercise ability.