摘要
【目的】明确贵州省水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性,为水稻纹枯病的科学防治及合理用药提供参考。【方法】采用菌丝生长速率法测定从贵州省4个地区采集分离的52株水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性及药剂间的相关性。【结果】供试菌株对丙环唑的EC50值为0.058~2.381μg·mL-1,均值为0.422μg·mL-1;供试菌株对吡唑醚菌酯的EC50值范围从0.077~0.936μg·mL-1,均值为0.313μg·mL-1。抗性水平分析表明,湄潭地区的水稻纹枯病菌对丙环唑和吡唑醚菌酯出现了低抗水平的菌株,抗性频率分别为16.7%、13.3%。聚类分析表明,水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性差异与菌株地理来源无明显相关性,且这两种药剂的敏感性之间也无显著相关性。【结论】供试菌株中多数菌株对丙环唑和吡唑醚菌酯的敏感性较高。药剂间未产生交互抗性,生产上可以交替使用。
【Objective】 Sensitivities of Rhizoctonia solani on rice to propiconazole and pyraclostrobin were determined for proper pesticide application on the disease control in Guizhou.【Method】 Mycelium growth rate was used as the criterion for testing the pesticide sensitivities of 52 strains of R. solani collected from 4 regions in the province.【Result】 The EC50 of propiconazole on the pathogens ranged from 0.058 μg·mL-1 to 2.381 μg·mL-1, averaging 0.422 μg·mL-1. That of pyraclostrobin were between 0.077 μg·mL-1 and 0.936 μg·mL-1, averaging 0.313 μg·mL-1. Strains of low resistance to the pesticides were found in Meitan area of the province with a resistance frequency of 16.7% on propiconazole and 13.3% on pyraclostrobin. A cluster analysis showed no significant correlation between the pesticide sensitivities and the geographical origins of the pathogens, nor between the two pesticides.【Conclusion】 A large number of the R. solani isolates in the study were highly sensitive to propiconazole and pyraclostrobin, and no apparent cross-resistance observed between the two fungicides. Hence,alternative application of them for disease control was deemed appropriate.
作者
刘世江
丁怡
赵琪君
李明
文小东
宋星陈
李荣玉
LIU Shi-jiang;DING Yi;ZHAO Qi-jun;LI Ming;WEN Xiao-dong;SONG Xing-chen;LI Rong-yu(Institute of Crop Protection,Guizhou University,Guiyang,Guizhou 550025,China;Engineering and Technology Research Center of Kiwifruit,Guiyang,Guizhou 550025,China;Provincial Key Laboratory for Agricultural Pest Management in Mountainous Region,Guizhou University,Guiyang,Guizhou 550025,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第11期1294-1301,共8页
Fujian Journal of Agricultural Sciences
基金
国家重点研发计划项目(2016YFD0200500)
国家自然科学基金(31701816)
贵州省科学技术基金计划项目(黔科合基础[2017]1083)
贵州大学引进人才科研项目[贵大人基合字(2015)07号)]
黔科合平台人才项目([2017]5788号)
关键词
纹枯病菌
丙环唑
吡唑醚菌酯
敏感性
交互抗性
Rhizoctonia solani
propiconazole
pyraclostrobin
pesticide sensitivity
cross-resistance