麻黄-甘草药对抑制过敏性哮喘的效应及机制初探

Effects and Mechanism of Mahuang-Gancao Couplet Medicines on Allergic Asthma

目的研究麻黄-甘草药对对过敏性哮喘的影响,并初步探讨其作用机制。方法建立屋尘螨(HDM)诱导的过敏性哮喘模型,造模同时给予麻黄-甘草药对提取物(MG)。使用无创肺功能仪检测小鼠气道反应性,HE染色观察小鼠肺组织病理学变化,Masson染色观察小鼠肺组织的胶原沉积情况,进行血液嗜酸性粒细胞(EOS)计数,ELISA法检测支气管肺泡灌洗液(BALF)及肺组织IL-4、IL-5、IL-13、TGF-β1及血清IgE的表达,免疫组化检测肺组织E-cadherin、N-cadherin、Vimentin、α-SMA和TGF-β1蛋白表达情况。结果 MG可显著降低气道高反应性,抑制BALF及肺组织IL-4、IL-5和IL-13的表达,降低血液EOS及血清IgE的表达,并且减轻肺组织的炎症浸润及胶原沉积。进一步结果表明MG可抑制肺组织TGF-β1的表达,上调E-cadherin以及下调N-cadherin、Vimentin、α-SMA的表达。结论 MG能够通过抑制支气管上皮间质转化,从而缓解气道的炎症状态以及改善哮喘的气道重塑,进而发挥对过敏性哮喘的治疗作用。

OBJECTIVE To investigate the effect and mechanism of Mahuang-Gancao couplet medicines(MG)on allergic asthma.METHODS The airway responsiveness was detected by non-invasive pulmonary function test.The pathological changes of lung tissue were observed by HE staining.The collagen deposition of lung tissue was observed by Masson staining.The eosinophil(EOS)count in blood was performed.IL-4,IL-5,IL-13,TGF-β1 and IgE were detected by ELISA assay.The expression of E-cadherin,N-cadherin,Vimentin,α-SMA and TGF-β1 were detected by immunohistochemistry.RESULTS MG significantly reduced airway hyperresponsiveness,inhibited the expression of IL-4,IL-5 and IL-13 in BALF and lung tissue,decreased the expression of serum IgE,and alleviated inflammation of lung tissue.MG inhibited the expression of TGF-β1 in lung tissue,increased the expression of E-cadherin and down-regulated the expression of N-cadherin,Vimentin and α-SMA.CONCLUSION MG can play a key role in the treatment of allergic asthma by inhibiting the bronchial epithelial-mesenchymal transition,thereby alleviating the inflammatory state of the airway and improving the airway remodeling of asthma.