菌落的等效性模拟及标准物质研究

Equivalent Simulation and Reference Material of the Bacterial Colonies

菌落本身不稳定,不方便携带,不适合用作菌落计数器计量校准用标准物质。采用菌落的模拟图像来代表菌落,根据菌落的特点,将大小不一、分布不均的菌落图像加工成图片,等效模拟菌落总数和大小研制成模拟菌落标准物质。采用分层随机抽样的方法,进行均匀性和稳定性的研究,结果表明制备的模拟菌落标准物质具有良好的均匀性和足够的稳定性。通过人工计数和影像测量机对模拟菌落的总数、大小进行了定值并评定了不确定度。该系列标准物质便于存放、携带,已通过国家二级标准物质评审,编号为GBW(E)130586~GBW(E)130590,可用于菌落计数器的校准、分析方法评价等方面。

The bacterial colony itself is unstable and inconvenient to carry,so it is not suitable for use as a reference material for calibration of colony counters.Colony simulation images were used to represent colonies.According to the characteristics of colonies,colony images of different sizes and uneven distribution were processed into pictures,and the total number and size of simulated colonies were simulated to simulate standard colony materials.The total number and size of the simulated bacterial colony were equivalent to the simulated bacterial colony reference materials.A stratified random sampling method was used to study the homogeneity and stability of the reference materials.The results showed that the prepared reference materials had good homogeneity and stability.Through rigorous calculation and data processing,the total number and size of the simulated bacterial colony were determined and the uncertainty was calculated.The series of reference materials could be stored and carried easily,so they were suitable for bacterial colony counter calibration and analysis method evaluation,and have been certified as the secondary national certified reference materials with the number of GBW(E)130586~GBW(E)130590.