摘要
目的:探讨白藜芦醇(Res)联合奥沙利铂(Oxa)对人结肠癌细胞恶性生物学行为的抑制作用及机制。方法:将体外培养结肠癌HCT-116细胞分为Res组、Oxa组、联合组及对照组,分别以100μmoL/L Res、2μmoL/LOxa、100μmoL/L Res+2μmoL/LOxa、等量生理盐水处理,各组分别处理24、36、48 h。采用噻唑蓝(MTT)法检测结肠癌HCT-116细胞增殖抑制情况;采用Transwell小室实验检测结肠癌HCT-116细胞侵袭能力;Wound Healing法检测结肠癌HCT-116细胞迁移能力;采用蛋白免疫印迹法检测结肠癌HCT-116细胞波形蛋白(Vimentin)蛋白表达情况。结果:干预48 h时,Res组、Oxa组及联合组结肠癌HCT-116细胞增殖抑制率分别为(28.41±6.25)%、(36.11±8.47)%、(83.61±12.72)%;干预48 h时,对照组、Res组、Oxa组及联合组结肠癌HCT-116细胞侵袭率分别(97.14±2.81)%、(81.23±4.52)%、(69.84±3.97)%、(37.11±3.58)%;迁移率分别为(68.57±4.02)%、(46.11±3.13)%、(31.25±2.86)%、(18.79±2.21)%;Vimentin蛋白相对表达量分别为0.93±0.14、0.52±0.08、0.31±0.04、0.13±0.02。Res组、Oxa组及联合组结肠癌HCT-116细胞增殖抑制率呈逐渐升高趋势(P<0.05);与对照组比较,Res组、Oxa组及联合组结肠癌HCT-116细胞侵袭率、迁移率和Vimentin蛋白相对表达量降低,且联合组显著低于Res组与Oxa组,差异有统计学意义(P<0.05)。结论:Res与Oxa联合使用具有显著的协同效应,可更有效抑制结肠癌HCT-116细胞增殖,并降低其侵袭和迁移能力,其机制可能与下调Vimentin蛋白表达有关。
Objective:To investigate the inhibitory effect and mechanism of resveratrol(Res)combined with oxaliplatin(Oxa)on the malignant biological behavior of human colon cancer cells.Methods:Colon cancer HCT-116 cells were cultured in vitro.The Res group,the Oxa group,the combination group and the control group were treated with 100μmoL/L Res,2μmoL/L Oxa and 100μmoL/L Res+2μmoL/L Oxa,the same amount of saline,and each group was treated for 24,36,48 h.The inhibition of proliferation of colon cancer HCT-116 cells was detected by MTT assay;Transwell chamber assay was used to detect the invasion ability of colon cancer HCT-116 cells;Wound healing method was used to detect the migration ability of colon cancer HCT-116 cells;The expression of Vimentin protein(vimentin)in colon cancer HCT-116 cells was detected by Western blotting(Wb)method.Results:At 48 h after intervention,the proliferation inhibition rates of HCT-116 cells in the Res group,the Oxa group and the combination group were(28.41±6.25),(36.11±8.47)and(83.61±12.72)%,respectively;At 48 h after intervention,the invasive rates of HCT-116 cells in the control group,the Res group,the Oxa group and the combination group were(97.14±2.81),(81.23±4.52),(69.84±3.97),(37.11±3.58)%,respectively;The mobility rates were(68.57±4.02),(46.11±3.13),(31.25±2.86),and(18.79±2.21)%,respectively;The relative expression levels of Vimentin protein were(0.93±0.14),(0.52±0.08),(0.31±0.04),and(0.13±0.02),respectively.The proliferation inhibition rate of colon cancer HCT-116 cells in the Res group,the Oxa group and the combination group increased gradually(P<0.05);Compared with the control group,the invasion rate,mobility rate and Vimentin protein expression of HCT-116 cells in the Res group,the Oxa group and the combination group significantly decreased,and the combination group was significantly lower than the Res group and the Oxa group(all P<0.05).Conclusion:The combination of Res and Oxa has significant synergistic effect,which can inhibit the proliferation of colon cancer HCT-116 cells and reduce its invasion and migration rate,and its mechanism may be related to the down-regulation of Vimentin protein expression.
作者
赵桂柱
孙占军
温志鹏
Zhao Guizhu;Sun Zhanjun;Wen Zhipeng(Department of Pharmacy,Baogang Hospital,Baotou 014010,China)
出处
《世界中医药》
CAS
2019年第12期3202-3205,共4页
World Chinese Medicine
基金
2019年内蒙古自治区自然科学基金项目(2019MS08181)