miR-21及其靶基因RECK、Smad7在心脏性猝死心肌组织中的表达及其意义

EXPRESSION AND SIGNIFICANCE OF MICRORNA-21 AND ITS TARGET GENES RECK AND SMAD7 IN MYOCARDIAL TISSUE IN SUDDEN CARDIAC DEATH

目的 观察微小RNA-21(miR-21)与伴有Kazal基序富含半胱氨酸的逆转诱导蛋白(RECK)、Smad7在心脏性猝死(SCD)心肌组织中的表达水平,并探讨其意义。方法 选择因SCD的20例心脏标本作为SCD组;选取20例死因为非心脏疾患的心脏标本作为对照组。应用免疫组织化学染色方法分别检测两组心肌组织中RECK和Smad7表达,采用荧光定量PCR方法检测两组心肌组织中miR-21、RECK和Smad7的表达。结果 免疫组化染色结果显示,两组心肌组织中RECK、Smad7的表达比较,差异均有统计学意义(Z=-4.008、-2.939,P<0.05);两组心肌组织中RECK与Smad7的表达水平呈正相关(r=0.706,P<0.05)。荧光定量PCR方法检测结果显示,SCD组心肌组织中miR-21与RECK、Smad7相对表达量均呈负相关(r=-0.608、-0.681,P<0.05)。结论 SCD心肌组织中miR-21高表达,RECK、Smad7低表达,miR-21与RECK、Smad7联合应用有望作为诊断SCD较为客观的指标。

Objective To investigate the expression and significance of microRNA-21(miR-21),RECK,and Smad7 in myocardial tissue in sudden cardiac death(SCD).Methods A total of 20 heart specimens from patients with SCD as SCD group,and 20 cardiac specimens from patients without heart disease were selected as control group.Immunohistochemical staining was used to mea-sure the protein expression of RECK and Smad7 in myocardial tissue,and quantitative real-time PCR was used to measure the mRNA expression of miR-21,RECK,and Smad 7 in myocardial tissue.Results Immunohistochemical staining showed that there was a significant difference in the expression of RECK and Smad7 in myocardial tissue between the two groups(Z=-4.008,-2.939,P<0.05),and there was a positive correlation between RECK and Smad7 in myocardial tissue(r=0.706,P<0.05).Quantitative real-time PCR showed that in the SCD group,the relative expression of miR-21 was negatively correlated with that of RECK and Smad 7(r=-0.608,-0.681,P<0.05).Conclusion There is high expression of miR-21 and low expression of RECK and Smad7 in myocardial tissue in SCD,and combined measurement of miR-21,RECK,and Smad7 can be used as an objective indicator for the diagnosis of SCD.