CD14、TLR4基因多态性与百色地区原住壮族人群脊柱结核易感关联性研究

Relationship between CD14 and TLR4 gene polymorphism and susceptibility of spinal tuberculosis in Baise indigenous Zhuang people

目的:研究脂多糖受体(CD14)和Toll样受体4(TLR4)基因多态性在壮族人群的分布特征及其与脊柱结核易感性的关系。方法:2011年1月至2017年12月期间右江民族学院附属医院就诊的百色地区原住壮族人群脊柱结核患者60例、肺结核患者60例和健康对照组60例三组人群,用A测序法测定三组人群的TLR4基因序列和CD14基因序列,比较三组人群CD14、TLR4基因多态位点的基因型频率和等位基因频率。结果:TLR4基因Asp299Gly和Thr399 Ile位点在壮族人群脊柱结核患者和肺结核患者均未发现呈多态性分布。壮族人群均发现CD14/-159呈多态性,以TT基因型为主,CD14基因C-159T和G-1145A等位基因频率按Hard-Weiber平衡吻合度检验,脊柱结核组、肺结核组和健康对照组三组含有T等位基因携带者的基因型频率和等位基因频率分布两两比较有统计学显著性差异(χ2=8.462,P=0.004;χ2=5.432,P=0.025;χ2=7.321,P=0.021)。结论:百色地区壮族脊柱结核患者和肺结核患者未发现TLR4基因Asp299Gly和Thr399Ile多态性;百色地区壮族人群脊柱结核患者和肺结核患者存在CD14/-159多态性,以TT为主,CD14基因-159TT和-1145AA基因型可能为百色地区原住壮族人群脊柱结核病的易感高风险因子。

Objective:To study the lipopolysaccharide receptor CD14 and toll-like receptor 4(TLR4)polymorphism in the distribution features of the Zhuang people and its relationship with spinal tuberculosis susceptibility.Methods:60 cases of patients with spinal tuberculosis,60 patients with tuberculosis and health control group 60 cases of people of indigenous Zhuang in Baise were taken study during January 2011 to December 2017 in affiliated hospital of Youjiang Medical University for Nationalities,TLR4 gene sequences and CD14 gene sequence were determined with A sequencing method in this three groups of people,CD14 and TLR4 gene polymorphism of genotype frequency and allele frequency in the three groups of people were compared.Results:The TLR4 gene Asp299Gly and Thr399Ile loci were not found polymorphism distribution in indigenous Zhuang people in patients with spinal tuberculosis and tuberculosis patients,CD14/-159 were found polymorphism in indigenous Zhuang people,give priority to with TT genotype,according to the balance Hard-Weiber alignment inspection,CD14 gene C-1145 and G-159T allele frequency were significant difference comparing two groups of people(χ2=8.462,P=0.004;χ2=5.432,P=0.025;χ2=7.321,P=0.021).Conclusion:There are not found TLR4 gene of Asp299Gly and Thr399Ile polymorphisms in patients with spinal tuberculosis and tuberculosis patients in Baise indigenous Zhuang.There are CD14/-159 polymorphism exist in patients with spinal tuberculosis and tuberculosis patients of indigenous Zhuang people in Baise region,and priority to with TT,CD14-159TT and CD14-1145AA genotype may be susceptible to high-risk factors of people with spinal tuberculosis in Baise indigenous Zhuang.