羟氯喹抑制系统性红斑狼疮Th17细胞的分化研究

Hydroxychloroquine inhibits Th17 cell differentiation in systemic lupus erythematosus

目的:研究羟氯喹对体外和SLE患者Th17细胞分化的影响。方法:将30例SLE患者依据用药不同分为两组进行治疗:分别为强的松组和羟氯喹+强的松组,采用ELISA和RT-PCR检测IL-17的表达,采用流式细胞仪方法测定羟氯喹治疗前后SLE患者Th17的变化;在体外实验中,将Th0在诱导Th17分化的条件下,加或不加HCQ进行培养,检测Th17细胞分化和IL-17表达情况。结果:SLE患者检测结果发现,与强的松组比较,羟氯喹+强的松组IL-17的水平、基因表达和Th17细胞比例显著降低。体外实验发现,羟氯喹可抑制Th17细胞的增殖和分化以及IL-17的产生。结论:羟氯喹可抑制SLE患者Th17细胞分化和IL-17的产生,为羟氯喹治疗SLE提供了依据。

Objective:To investigate the effect of HCQ on Th17 cells differentiation both in vitro and in vivo of patients with systemic lupus erythematosus.Methods:30 patients with SLE were divided into 2 groups:prednisone group and HCQ plus prednisone group.Interleukin 17(IL-17)expression was analyzed by ELISA and RT-PCR.Th17 were measured in patients with SLE by flow cytometry before and after treatment.In vitro,Th0 cells were cultured in Th17-inducing conditions with or without HCQ.Cell differentiation and IL-17 expression were analyzed.Results:In patients,HCQ plus prednisone treatment inhibited IL-17 production,gene expression,and Th17 cell differentiation.In vitro,HCQ inhibited Th17 cell proliferation and differentiation,as well as IL-17 production.Conclusion:HCQ inhibited Th17 cell differentiation and IL-17 production both in vitro and in patients with SLE.Our study provides additional evidence for HCQ as a treatment for SLE.