前列地尔联合针灸对椎动脉型颈椎病家兔血液流变学、一氧化氮、血浆内皮素的影响

Effects of Alprostadil Combined with Acupuncture on Hemorheology,NO and ET in Rabbits with VACS

目的探讨前列地尔注射液联合针灸对椎动脉型颈椎病兔模型椎-基底动脉血液流变学及血清一氧化氮(NO)、血浆内皮素(ET)含量的影响。方法选取健康成年雄性清洁级家兔80只,按随机数字表法分为空白模型组、模型组、针刀组、前列地尔组及联合组,各16只。除空白模型组外,其他各组均进行造模,并采用对应干预治疗。干预治疗4周后,对各组家兔进行经颅多普勒(TCD)检测,并比较家兔血流动力学、血液流变学改善情况,以及血清NO及血浆ET表达水平。结果建模后,模型组、针刀组、前列地尔组及联合组左侧椎动脉速度、右侧椎动脉速度、基底动脉血流速度血液流变学指标、血清NO及血浆ET表达水平均优于空白模型组(P <0. 05);治疗后,针刀组、前列地尔组及联合组以上指标均优于本组治疗前(P <0. 05),联合组以上指标明显优于针刀组及前列地尔组(P <0. 05),针刀组与前列地尔组比较无显著差异(P> 0. 05)。结论前列地尔注射液联合针灸可改善椎动脉型颈椎异常家兔的血流动力学及血液流变学,且能有效改善血清NO及血浆ET的表达水平,增加脑供血状态。

Objective To investigate the effects of alprostadil injection combined with acupuncture on vertebrobasilar hemorheology and serum nitric oxide( NO) and plasma endothelin( ET) levels in rabbit model of vertebro-arterial cervical spondylosis( VACS). Methods Eighty healthy adult male clean rabbits were selected and divided into the blank model group,model group,acupuncture group,alprostadil group,and combined group according to the random number table method,16 rabbits in each group. Except for the blank model group,the four other groups were modeled and treated with corresponding interventions. After 4 weeks of intervention,the rabbits underwent transcranial Doppler( TCD) detection,and the improvements of hemodynamics and hemorheology,serum NO expression level and plasma ET expression level were compared. Results After modelled,the hemorheological indexes such as the left vertebral artery velocity,right vertebral artery velocity and basilar artery blood flow velocity,as well as the expression levels of serum NO and serum ET in the model group,acupuncture group,alprostadil group,combined group were significantly better than those in the blank model group( P < 0. 05).After treatment,the indexes and levels in the acupuncture group,alprostadil group,and combined group were significantly better than before treatment( P < 0. 05);the improvements in the combined group were better than those in the acupuncture group and alprostadil group( P < 0. 05);there was no statistically significant difference between the acupuncture group and alprostadil group( P > 0. 05).Conclusion The combination of alprostadil with acupuncture can improve the hemodynamics and hemorheology in abnormal VACS in rabbits,effectively improve the expression levels of serum NO and plasma ET and increase the state of blood supply to the brain.