IL-1β基因单核苷酸多态性与足月新生儿败血症的相关性研究

Association between interleukin-1β single nucleotide polymorphisms and sepsis in full-term neonates

目的分析IL-1β基因单核苷酸多态性(SNP)与足月新生儿败血症的关系,为新生儿败血症的预防提供理论依据。方法选取昆明市儿童医院新生儿科2017年1-12月血培养阳性,确诊为败血症的50例新生儿为败血症组,有临床症状但血培养阴性,诊断为临床败血症的50例患儿为临床败血症组,另选取同期50例非感染新生儿作为对照组。采用Sequenom SNP测序技术检测IL-1β基因2个位点(rs1143627、rs1143643),比较三组间各等位基因频率和基因型频率的分布差异;采用多因素Logistic回归分析IL-1β基因型与新生儿败血症的关系。结果三组间IL-1β基因rs1143627(C/T)各基因型分布差异均无统计学意义(P>0.05);三组间IL-1β基因rs1143643(C/T)各基因型分布差异均有统计学意义(χ~2=15.94,P<0.05);三组间上述两个位点各等位基因分布差异无统计学意义(P>0.05)。Logistic回归分析显示胎龄与新生儿败血症的发生相关(OR=0.6,95%CI:0.418~0.915);性别、日龄、rs1143627和rs1143643基因多态性对新生儿败血症发病的影响差异无统计学意义。结论胎龄是新生儿败血症的影响因素。IL-1β基因位点rs1143627(C/T)和rs1143643(C/T)可能与新生儿败血症无相关性。

Objective To study the association between single nucleotide polymorphisms(SNP) in interleukin-1β(IL-1β) and sepsis in full-term neonates,in order to provide theoretical evidence for the prevention of neonatal sepsis. Methods A total of 50 neonates with positive result of blood culture and diagnosed with sepsis in Kunming Children′s Hospital were selected as sepsis group from January to December 2017,and 50 children with clinical symptoms and negative results of blood culture were selected in clinical sepsis group.Meanwhile,50 children without infection were in control group.The polymorphisms of IL-1β(rs1143627 and rs1143643) were analyzed using the Sequenom SNP,and the genotypic and allelic frequencies among three groups were compared.The relationship between IL-1β genotypes and sepsis was analyzed by Logistic regression. Results No significant differences were found on genotypic frequencies of IL-1β rs1143627(C/T)(P>0.05)among sepsis group,clinical sepsis group and control group.But the genotypic frequencies of IL-1β rs1143643(C/T) were significantly different among three groups(χ~2=15.94, P<0.05).Moreover,there were no significant differences on the allelic frequencies of the SNPs among the three groups(P>0.05).Logistic regression analysis indicated that gestational age was closely associated with neonatal sepsis(OR=0.6,95%CI:0.418-0.915),while the influences of gender,age,polymorphisms in rs1143627 and rs1143643 genes on neonatal sepsis were not significant(P>0.05). Conclusion Gestational age is the influencing factor of neonatal sepsis.IL-1β polymorphisms(rs1143627 and rs1143643) may not have any association with neonatal sepsis.