辣椒CaNAC45转录因子基因的克隆与表达分析

Cloning and Expression Analysis of CaNAC45 Transcription Factor Gene in Pepper

干制辣椒是一种重要的经济作物,其生产中受干旱、高盐、低温等非生物胁迫危害时,NAC (NAM,ATAF1/ATAF2,CUC2)转录因子可调控抗逆基因对胁迫进行抵抗。为研究干制辣椒CaNAC45转录因子的理化性质和其在非生物胁迫下的表达,本研究以2种不同耐盐能力的干制辣椒为材料克隆出CaNAC45转录因子基因。结果表明:两种材料的CaNAC45转录因子基因序列一致,全长为890 bp,编码290个氨基酸,理论等点为8.1,为碱性蛋白,存在3个无序化域。N端保守,其保守结构域在第8位氨基酸至第132位氨基酸之间,符合NAC家族成员特征。进化树分析表明CaNAC45转录因子属于非生物胁迫响应ATAF亚家族。CaNAC45转录因子三维结构预测表明,α螺旋结构占总蛋白6.9%,β片层结构占总蛋白的14.14%,无规则卷曲占总蛋白的78.97%。CaNAC45基因在高盐、干旱、ABA、SA和MeJA胁迫处理下表达量上调。说明这个基因可能在辣椒抗逆中起重要作用,本研究结果为研究NAC转录因子家族在干制辣椒抗逆中的调控功能提供理论基础。

Dry pepper is an important cash crop.When it is threatened by drought,high salt,low temperature and other abiotic stresses in production,NAC(NAM,ATAF1/ATAF2,CUC2)transcription factor can regulate the resistance gene to stress.In order to study the physicochemical properties of the dry pepper CaNAC45 transcription factor and its expression under abiotic stress,CaNAC45 gene was cloned from two kinds of dry pepper with different salt tolerance.The results showed that the gene sequences of CaNAC45 transcription factor were coincident in the two varieties.With a total length of 890 bp,encoding 290 amino acids,theoretical equivalent point of 8.1 and 3 disordered domains.Evolutionary tree analysis showed that CaNAC45 belonged to the ATAF subfamily of abiotic stress response.CaNAC45 transcription factor protein contained 6.9%ofα-helix,14.14%ofβ-sheet and 78.97%of random coil.The expression of dry pepper CaNAC45 gene increased in high-salt,drought,ABA,SA,MeJA stress.This result suggested that CaNAC45 gene might play an important role in the resistance to abiotic stresses in dry pepper.The expression of dry pepper CaNAC45 gene increased in various abiotic stresses.And this result provided a theoretical basis to study the regulatory function of NAC transcription factor family in resistance of dry pepper.