摘要
多花兰(Cymbidium floribundum)为兰科(Orchidaceae)兰属(Cymbidium)多年生草本植物,具有极高观赏价值。本研究以多花兰的营养器官(根,假鳞茎,叶片)和花器官(萼片,捧瓣,唇瓣和合蕊柱)为材料,使用实时荧光定量PCR技术对5个备选内参基因(18SrRNA,28SrRNA,Actin,GAPDH和25SrRNA)进行扩增,结合NormFinder、geNorm和Bestkeeper这3个软件分析各内参基因的稳定性,筛选出合适的内参基因。结果显示,在多花兰营养器官和花器官中不同内参基因表达稳定性有一定差别,综合3个软件的分析结果表明多花兰的花器官和营养器官最合适内参基因分别是GAPDH和28SrRNA。
Cymbidium floribundum is a perennial herb of the genus Cymbidium belongs to the Orchidaceae family,it has a high ornamental value.In this study,the vegetative organs(root,pseudobulb and leaf)and the floral organs(sepal,petal,lip and gynostemium)of C.floribundum were used as materials,and five alternative reference genes(18SrRNA,28SrRNA,Actin,GAPDH,25SrRNA)were amplified by RT-qPCR.The stability of each reference gene was analyzed by NormFinder,geNorm and Bestkeeper,in order to screen out the appropriate reference genes.The results showed that there were some differences in the expression stability of different reference genes in different organs of C.floribundum.The most suitable reference genes for floral organs and vegetative organs are GAPDH and 28SrRNA.
作者
张玥
陈娟
谢泰祥
陈明堃
周杰
艾叶
Zhang Yue;Chen Juan;Xie Taixiang;Chen Mingkun;Zhou Jie;Ai Ye(Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization,College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,350002)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第24期8163-8169,共7页
Molecular Plant Breeding
基金
福建省自然科学基金项目(2016J01703)
国家自然科学基金项目(31700618)
福建省种业创新与产业化工程项目(ZYCX-LY-2017005)
福建农林大学创新项目(KFA17331A)共同资助
