小豆矮秆窄叶突变体转录组分析和激素应答反应

Transcriptome analysis and determination of hormone response of a narrow leaf and dwarf mutant of adzuki bean

【目的】株型是影响作物产量的重要性状,对小豆矮秆窄叶突变体nld进行转录组学分析,以期探究小豆矮秆窄叶的转录水平调控机制。【方法】构建小豆栽培种野生型GM437和矮秆窄叶突变体nld的根、茎、叶转录组文库,利用RNA-Seq进行转录组学分析,对得到的差异表达基因进行GO注释和KEGG富集分析;同时,利用外施激素的方法确定突变体应答激素种类。【结果】转录组分析发现,差异表达基因主要集中于植物激素信号转导通路和苯丙烷生物合成通路。外施赤霉素可部分恢复矮秆窄叶突变体的生长。关键基因4-香豆酸辅酶A连接酶、肉桂酰辅酶A还原酶、肉桂醇脱氢酶、黑酸叶绿醇转移酶、4-羟苯基丙酮酸双氧酶等在小豆植株发育过程中起到重要作用。【结论】植物激素信号转导通路和苯丙烷生物合成通路为小豆矮秆窄叶建成主要调控通路,赤霉素在突变体植物激素信号转导通路中起主要作用。

【Objective】Plant architecture is an important trait affecting crop yield.To reveal the molecular mechanism of the narrow leaf and dwarf mutant nld in transcription,transcriptome analysis was performed on the narrow leaf and dwarf mutant of adzuki bean.【Methods】Transcriptome libraries of wild type GM437 and mutant nld were constructed and sequenced by RNASeq.GO annotation and KEGG enrichment of differentially expressed genes were performed.The types of response hormone were confirmed using exogenous hormone.【Results】Transcriptome analysis showed that the differentially expressed genes were mainly annotated in plant hormone signal transduction pathway and phenylpropane biosynthesis pathway.Exogenous gibberellin was able to partly recover the growth of nld mutant.4 CL,CAD,CCR,HPTand HPDgenes play an important role in the plant development of adzuki bean.【Conclusion】The phenylpropane biosynthesis pathway and plant hormone signal transduction pathway are the main regulating pathway of narrow leaf and dwarf in adzuki bean.Gibberellin is a main factor in the plant hormone signal transduction pathway of nld mutant.