高尿酸血症患者黄嘌呤氧化酶活性及影响因素分析

Analysis of xanthine oxidase activity and influencing factors in patients with hyperuricemia

目的对比高尿酸血症患者与健康人的黄嘌呤氧化酶(xanthine oxidase,XO)活性之间的差异及其影响因素。方法选择高尿酸血症男性患者129例为高尿酸血症组,其中痛风112例、单纯高尿酸血症17例;同时随机选择同期男性健康志愿者95名为对照组。采用酶联免疫吸附测定(ELISA)方法测定血清XO活性;采用独立样本t检验进行两两比较,采用多元线性回归分析独立影响因素。结果与正常对照组相比,高尿酸血症患者的XO活性与健康志愿者相比升高,且差异有统计学意义[(159.6±4.0对138.7±7.5)U/L,P<0.05];相关分析表明高尿酸血症患者XO活性与体重指数、腰臀比、舒张压、总胆固醇、血尿酸、肌酐、空腹血糖、尿酸清除率显著相关。逐步多元回归分析中,以XO活性为因变量,结果表明腰臀比、尿酸清除率是XO活性的影响因素。结论高尿酸血症患者XO活性升高,肥胖尤其是腹型肥胖是XO活性的独立危险因素;提示改善腹型肥胖可能会对高尿酸血症患者血尿酸水平的控制起到积极作用。

Objective To evaluate and compare the difference of xanthine oxidase(XO)activity between patients with hyperuricemia and healthy people,and to analyze the influencing factors.Methods A total of 129 male patients with hyperuricemia were selected to hyperuricemia group,including 112 patients with gout and 17 patients with hyperuricemia alone.Simultaneously,95 male healthy volunteers were randomly selected as the control group.Serum XO activity was determined by enzyme-linked immunosorbent assay(ELISA).Independent t-test was used for pairwise comparison,and multivariate linear regression analysis was used to analyze independent influencing factors.Results Compared with the normal control group,the XO activity of patients with hyperuricemia showed a significant increase[(159.6±4.0 vs 138.7±7.5)U/L,P<0.05].Correlation analysis showed that XO activity positively associated with body mass index,waist-to-hip ratio,diastolic blood pressure,total cholesterol,serum uric acid,creatinine,fasting blood glucose,and uric acid clearance rate level.In the stepwise multivariate regression analysis,XO activity was used as the dependent variable.The results showed that waist-to-hip ratio and uric acid clearance rate were the influencing factors of XO activity.Conclusion Hyperuricemia patients showed elevated XO activity,with obesity,especially abdominal obesity,an independent risk factor for XO activity;suggesting that controlling of abdominal obesity may play a positive role in the treatment of hyperuricemia.