姬菇多糖对免疫低下型小鼠肝脏和肾脏抗氧化作用的影响

Effects of Acid Polysaccharides from Pleurotus cornucopiae on the Antioxidation in Mice with Immunosuppression

以姬菇的酸性多糖成分(APPC)为试验材料,建立环磷酰胺诱导的免疫抑制小鼠模型,测定相关抗氧化指标,探究APPC对小鼠肝脏、肾脏免疫活性作用的影响.将72只小鼠随机分为空白组、阳性组、模型组以及APPC高、中、低不同剂量组.通过检测各组肝脏和肾脏中超氧化物歧化酶(SOD)活性、谷胱甘肽氧化物酶(GSH-Px)活性、过氧化氢酶(CAT)活性、谷胱甘肽(GSH)含量和丙二醛(MDA)含量5项抗氧化指标,考察肝脏、肾脏功能免疫特性的变化.与模型对照组相比,APPC可以显著提高肝脏和肾脏的SOD活性、GSH-Px活性、CAT活性和GSH含量(P<0.01),降低肝脏和肾脏的MDA含量(P<0.01). APPC能提高免疫低下型小鼠肝脏免疫能力,改善肾脏的免疫调节能力.

By using acid polysaccharides from Pleurotus cornucopiae( APPC) as a test material,we established a cyclophosphamide-induced immunosuppressive mouse model and determine the relevant antioxidant index to explore the effect of APPC on the immune activity of liver and kidney in mice. The 72 mice were randomly divided into normal control group,model group,positive control group,as well as high,medium and low APPC dosage group. And by detecting the following antioxidant indexes,including superoxide dismutase( SOD) activity,glutathione( GSH) content,glutathione peroxidase( GSH-Px) activity,catalase( CAT) activity and malondialdehyde( MDA) content,the results could investigate the changes of liver and kidney function characteristics. Compared with the model group,APPC significantly increased SOD activity,GSH-Px activity,CAT activity and GSH content in liver and kidney( P<0.01),and decreased MDA content in liver and kidney( P< 0. 01). This implies that APPC has a certain improving effect on the immune function of the immune-inhibited mice liver,while betters the immune regulation ability of kidney.