苏姜猪线粒体基因组全长测序及线粒体基因多样性分析

Whole Genome Sequencing and Genetic Diversity of Mitochondrial Genome of Sujiang Pig

从分子水平上探究苏姜猪的遗传多样性,采用二代测序方法测定了苏姜猪线粒体基因组全序列,整合NCBI数据库中已公布的家猪类,以及本实验室测定的苏姜猪线粒体基因组全序列,使用蛋白编码基因和rRNA基因联合的数据集,基于贝叶斯法(BI法)和最大似然法(ML法)构建了家猪类的系统发生树,测序结果表明,该样本线粒体基因组全长15 436 bps,包含典型的家猪类线粒体基因组37条基因,结构紧凑,其线粒体基因组碱基组成(A+T)约占40%,(C+G)约占60%,t RNA总共有8处错配,结果表明苏姜猪具有丰富的遗传多样性,江海型线粒体核苷酸多态性高于全国6个类型猪的平均水平,体现了其独特的遗传多样性.在基于线粒体基因组完整编码区的系统发育分析中,苏姜猪在中国6个类型家猪中单独成为一支,支持其作为一个新猪种而存在.

To discover the genetic diversity of Sujiang pig at molecular level,the complete mitochondrial genome of Sujiang pig was sequenced by next generation sequencing. The Sujiang pig had high genetic diversity in 15436 bps 37 genes,which A + T estimated percentage of 40%,C + G estimated percentage of 60% of its mitochondrial genome and t RNA had 8 mismatches. The Jianghai type mitochondrial nucleotide polymorphism was higher than the average level of the all six pig types,which revealed its unique genetic diversity. Phylogenetic analysis based on the complete coding region of the mitochondrial genome using NJ method,Sujiang pig was a separate pig among the six types of domestic pigs in China,and support its unique pig pedigree.