TRPM7干扰载体修复低氧/复氧致大鼠心肌细胞系H9C2损伤

TRPM7 interference vector repaires injury caused by hypoxia/reoxygenation in cardiomyocyte cell line H9C2

目的研究瞬时受体电位通道7(TRPM7)干扰载体对低氧大鼠心肌细胞系(H9C2)修复功能的影响及其机理。方法建立H9C2心肌细胞低氧/复氧模型,构建TRPM7干扰表达载体转染H9C2细胞,用RT-qPCR与Western blot检测H9C2细胞低氧诱导因子(HIF1-α)和TRPM7的表达,流式细胞计量术检测胞内钙离子水平([Ca^2+]i)和细胞凋亡,确定TRPM7对H9C2心肌细胞的修复作用。结果H9C2细胞转染TRPM7干扰载体后,细胞的HIF1-α和TRPM7表达显著下降(P<0.05),[Ca^2+]i降低及凋亡率减少(P<0.05)。结论TRPM7干扰载体可能通过PI3K/Akt通路对低氧H9C2心肌细胞有显著的修复作用。

Objective To study the repair function and mechanism of TRPM7 interference vector in hypoxic myocardial cell line(H9C2).Methods The hypoxia/reoxygenation model of H9C2 cardiomyocytes was established and the TRPM7 interference expression vector was constructed to transfect H9C2 cells.The expression of HIF1-αand TRPM7 in H9C2 cells was detected by RT-qPCR and Western blot.[Ca^2+]i and apoptosis was determined by flow cytometry.Results When H9C2 cells were transfected with TRPM7 interference vector,the expression of HIF1-αTTRPM7,the[Ca^2+]i concentration and the apoptosis rate all decreased.Conclusions TRPM7 interference vector has significant repairing effect on hypoxia H9C2 cardiomyocytes,and the mechanism may be mediated PI3K/Akt pathway.