产妇血清ACA和抗β2-GP1抗体与不良妊娠结局的相关分析

Analysis between Maternal Serum ACA and Anti-β2-GP1 Antibodies and Adverse Pregnancy Outcomes

目的:探讨产妇血清抗心磷脂抗体(ACA)和抗β2-糖蛋白1(β2-GP1)抗体与不良妊娠结局的关系。方法:选取不良妊娠产妇130例作为研究组,同期正常分娩产妇130例作为对照组;采用酶联免疫吸附法(ELISA)检测2组产妇血清ACA及各亚型(ACA-IgA、ACA-IgG、ACA-IgM)、β2-GP1抗体及各亚型(抗β2-GP1-IgA、抗β2-GP1-IgG、抗β2-GP1-IgM)水平,并分析这些指标与围产儿死亡的相关性。结果:研究组产妇血清ACA-IgG与ACA-IgM阳性率明显高于对照组(P<0.01),抗β2-GP1-IgG及β2-GP1-IgM阳性率明显高于对照组(P<0.01);研究组产妇血清ACA、抗β2-GP1抗体及联合检查阳性率均明显高于对照组,差异均具有统计学意义(P<0.01);研究组产妇血清抗ACA-IgA、抗β2-GP1-IgA、抗β2-GP1-IgM抗体水平与围产儿死亡具有相关性(r=0.3126、P=0.018,r=0.3078、P=0.007,r=0.4012、P=0.021)。结论:血清ACA各亚型及抗β2-GP1各亚型抗体检测可用于评估不良妊娠结局。

Objective:To investigate the relationship between maternal serum anticardiolipin antibody(ACA)and anti-β2-GP1 antibody(β2-GP1)and adverse pregnancy outcomes.Methods:130 cases of adverse pregnancy were selected as study group,and during the same period,130 cases of normal delivery were used as control group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of the two groups of maternal serum ACA and its subtypes(ACA-IgA,ACA-IgG and ACA-IgM),β2-GP1 antibodies and the level of subtypes(anti-β2-GP1-IgA,anti-β2-GP1-IgG,anti-β2-GP1-IgM)to analyze the correlation between these indicators and perinatal mortality.Results:The positive rate of serum ACA-IgG and ACA-IgM in the study group was significantly higher than that in the control group(P<0.01),and the anti-β2-GP1-IgG and positive rate ofβ2-GP1-IgM was significantly higher than that of control group(P<0.01).The positive rate of serum ACA,anti-β2-GP1 antibody and combination test were significantly higher than those of control group,and the differences were statistically significant(P<0.01).The serum levels of anti-ACA-IgA,anti-β2-GP1-IgA and anti-β2-GP1-IgM were correlated with perinatal death in the study group(r=0.3126,P=0.018,r=0.3078,P=0.007,r=0.4012 and P=0.021).Conclusion:serum ACA subtypes and anti-β2-GP1 subtype antibody detection can be used to evaluate the outcomes of adverse pregnancy.