胫骨横向骨搬移技术联合外用象皮生肌膏对糖尿病足兔溃疡愈合影响的实验观察

Experimental Observation of the Effects of Transverse Tibial Bone Removal Technology and External Application of Xiangpi Shengji Ointment on Healing Diabetic Foot Ulcer in Rabbits

目的采用胫骨横向骨搬移技术联合外用象皮生肌膏治疗糖尿病足兔溃疡,观察其溃疡愈合速度。方法将32只新西兰大白兔随机分成4组,每组8只,分别是模型组(A组)、象皮生肌膏组(B组)、横向骨搬移组(C组)、横向骨搬移+象皮生肌膏组(D组),通过高脂高糖饲料喂养后用四氧嘧啶溶液耳缘静脉注射诱导糖尿病模型,再用磁片压迫法建立糖尿病足溃疡模型,A组络合碘换药,B组象皮生肌膏换药,C组骨搬移法治疗,D组骨搬移法联合象皮生肌膏换药治疗,于第1天和第14天分别测量溃疡面积大小。结果通过四氧嘧啶溶液注射后实验兔出现精神倦怠、体质量减轻,血糖明显升高,证明糖尿病造模成功;干预14 d后,与A组比较,B、C组实验兔溃疡面积明显减小(P<0.01);与B组比较,C组实验兔溃疡面积有所减小(P<0.05);与C组比较,D组实验兔溃疡面积显著减小(P<0.01)。结论胫骨横向骨搬移技术联合外用象皮生肌膏能明显促进兔糖尿病足溃疡的愈合,值得临床推广。

Objective To treat diabetic foot ulcers in rabbits by tibia transverse bone transfer technique combined with external application of Xiangpi Shengji ointment,and to observe the ulcer healing rate of rabbits in each group.Methods A total of 32 New Zealand white rabbits were randomly divided into 4 groups,with 8 rabbits in each group,which were a model group(group A),a Xiangpi Shengji Ointment group(group B),a transverse bone transfer group(group C),and a transverse bone transfer+Xiangpi Shengji Ointment group(group D).After feeding with high-fat and high-sugar feed,diabetic model was induced by intravenous injection of tetraoxopyrimidine solution in the ear margin,and diabetic foot ulcer model was established by magnetic disk compression method.Group A received dressing change of complex iodine.Group B received dressing change of Xiangpi Shengji Ointment.Group C was treated with bone removal method.Group D was treated with bone removal method combined with dressing change of Xiangpi Shengji Ointment.The ulcer area was measured on day 1 and 14 respectively.Results After the injection of alloxan solution,the experimental rabbits showed mental burnout,weight loss,and significantly increased blood sugar.The rabbit diabetes model was successfully built;After 14 days of intervention,compared with group A,the ulcer area of group B and group C was significantly reduced(P<0.01);Compared with group B,the area of ulcer in group C decreased(P<0.05);Compared with group C,the area of ulcer in group D decreased significantly(P<0.01).Conclusion Tibia transverse bone removal technique combined with external application of Xiangpi Shengji Ointment can significantly promote the healing of diabetic foot ulcer in rabbits,which is worthy of clinical promotion.