miR-21通过调控IL-6表达水平参与脑卒中复发研究

Research of participation of miR-21 in stroke recurrence by regulating IL-6 expression level

目的探讨脑卒中患者血清IL-6和miR-21水平对预后的影响。方法选择急性脑梗死患者作为急性脑梗死组(ACI组)。对照组为同时期的正常健康体检者。双抗体夹心抗体酶联免疫吸附试验 (ELISA)法检测ACI组和对照组的血清IL-6和STAT3的表达水平。实时定量PCR检测ACI组和对照组血清中miR-21的含量。统计分析脑卒中患者血清IL-6、STAT3及miR-21水平与复发是否存在相关性。结果 ACI患者血清miR-21和IL-6水平在发病1d内迅速升高,两者波动趋势一致。miR-21和IL-6的峰值水平越高,ACI患者1年内复发率越高。结论 miR-21通过调控STAT3作用于IL-6的表达,与ACI患者的复发密切相关。

Objective To investigate the effect of serum IL-6 and miR-21 on the prognosis of stroke patients.Methods The medical records and serum of patients with acute cerebral infarction were collected in ACI group.The control group were healthy persons who underwent physical examination at the same time. The expression levelsof serum IL-6 and STAT3 in ACI group and the control group were detected by double antibody sandwich antibodyenzyme-linked immunosorbent assay(ELISA). Real-time quantitative PCR was used to detect the content of miR-21 in the serum of ACI group and the control group. Statistical analysis was performed to determine whether serumIL-6 and STAT3 levels were correlated with serum miR-21 level and recurrence in stroke patients. Results SerummiR-21 and IL-6 levels in patients with ACI increased rapidly within 1 day of onset, and the fluctuation trend wasconsistent. The higher the peak levels of miR-21 and IL-6, the higher the recurrence rate of patients with ACIwithin 1 year. Conclusion miR-21 acts on the expression of IL-6 by regulating STAT3, which is closely related tothe recurrence of patients with ACI.