摘要
采用连续转接驯化,对某铀矿山酸性矿坑水分离纯化得到的嗜酸氧化亚铁硫杆菌(A.f)进行了耐Cu 2+、Mn 2+、Cr 3+和Pb 2+的驯化试验,考察了该菌株对这些重金属离子的耐受限值及氧化活性。试验结果表明:Cu 2+、Mn 2+、Cr 3+和Pb 2+对该菌株的生长抑制作用由大到小的排序为Cr 3+>Pb 2+>Cu 2+>Mn 2+;50%接种比例的连续转接驯化可以显著提高A.f对重金属离子的耐受限值;驯化后的A.f对Cr 3+、Pb 2+、Cu 2+和Mn 2+的耐受限值分别为2.1、3.5、34.0、45.0 g/L。
The continuous transfer domestication test was carried out to increase the tolerance of Acidthiobacillus ferrooxidans(A.f)which was separated and purified from acid mine water of a uranium mine to Cu 2+,Mn 2+,Cr 3+and Pb 2+,and its tolerance limits and oxidation activity to the heavy metal ions were investigated.The results show that these heavy metal ions follow the order of Cr 3+>Pb 2+>Cu 2+>Mn 2+in terms of their inhibitory effects on the growth of the strain;the continuous transfer domestication significantly increased the strain's tolerance limits to the heavy metal ions at 50%inoculation ratio;and the tolerance limits of A.f to Cr 3+,Pb 2+,Cu 2+and Mn 2+were 2.1,3.5,34.0 and 45.0 g/L,respectively.
作者
刘玉龙
李广悦
胡南
李建东
丁德馨
LIU Yu-long;LI Guang-yue;HU Nan;LI Jian-dong;DING De-xin(Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang 421001,China;China General Nuclear Power Group(CGN)Uranium Resources Co.,Ltd.Beijing 100029,China)
出处
《铀矿冶》
CAS
2020年第1期27-32,共6页
Uranium Mining and Metallurgy
基金
国家自然科学基金项目(50774047)
湖南省科技厅资助项目(2018CT5009)
关键词
嗜酸氧化亚铁硫杆菌
驯化
重金属
耐受性
Acidthiobacillus Ferrooxidans
domestication
heavy metal
tolerance
