基于16S rDNA高通量测序技术分析北京豆汁儿微生物多样性和功能预测的研究

Analysis of Microbial Diversity and Functional Prediction of Douzhir Based on 16S rDNA High-throughput Sequencing Technology

以北京不同城区具代表性的6家豆汁儿店的豆汁儿为研究对象,采用16S rDNA测序分析对豆汁儿中所含优势菌进行研究。结果表明:乳杆菌属(Lactobacillus)是豆汁儿中的优势菌属,乳杆菌目(Lactobacillales)占比达到95.8%以上。优势菌种为食窦魏斯氏菌(Weissella cibaria)、沙克乳酸杆菌(Lactobacillus sakei)。对豆汁儿中乳杆菌目(Lactobacillales)进行功能预测分析,推测豆汁儿中的风味物质来源于氨基酸代谢,发酵过程中产生β-低聚半乳糖及GOS,益于肠道菌群健康。

The dominant bacteria in Douzhir were studied by 16 S rDNA sequencing in six representative Douzhir shops in different urban areas of Beijing.The results showed that Lactobacillus was the dominant bacteria in Douzhir,and the proportion of Lactobacillales was more than 95.8%.The dominant strains were Weissella cibaria and Lactobacillus sakei.The function of Lactobacillales in Douzhir was predicted and analyzed.It was speculated that the flavor substances in Douzhir originated from amino acid metabolism.The production of beta-galacto-oligosaccharides and GOS during fermentation was beneficial to the health of intestinal flora.