Doehlert设计法结合渴求函数优化党参中多成分的提取工艺

Doehlert Design Combined with Desirability Function to Optimize the Extraction of Multi-components in Codonopsis pilosula

以党参中可溶性糖、紫丁香苷、党参炔苷、浸出物的含量为考察指标,不同料液比、提取时间、乙醇浓度、超声功率为影响因素,采用Doehlert设计法进行试验,结合渴求函数,建立数学模型,并经计算机辅助计算,确定最佳提取工艺。结果表明,回归模型与试验结果具有显著相关性,预测值与实际值较为接近。通过软件拟合结合实际,得出最佳工艺条件为:料液比1∶60,提取时间80 min,乙醇浓度70%,超声功率250 W,经工艺验证,实测渴求函数D=0.91,与预测值偏差较小。Doehlert设计法结合渴求函数优化党参中多成分的提取切实可行。该工艺下提取,可溶性糖434.14 mg/g,紫丁香苷86.37μg/g,党参炔苷287.94μg/g,浸出物65.11%,总体提取率高、操作简便,使用试剂简单经济,具有较高可操作性。

With soluble sugar,syringin,lobetyolin and extract contents of Codonopsis pilosula as investigation indexes,the different solid-lvquid ratio,extraction time,ethanol ratio and ultrasonic power as influencing factors,experiments were carried out by Doehlert design.A mathematical model was established based on desirability function.The optimum extraction process was determined by computer-aided calculation.Results showed that:The regression model showed a significant correlation with the experimental results,and the predicted values were close to the actual values.Through software fitting combining with practice,the optimum process conditions were obtained as follows:Solid-liquid ratio 1∶60,extraction time 80 min,ethanol ratio 70%,ultrasonic power 250 W.The process validation demonstrated that the measured values was 0.91,and the deviation between the measured values and the predicted values was small.Doehlert design combined with desirability function to optimize the extraction of multiple indexes in Codonopsis pilosula was practical and feasible.Under this process,the soluble sugar was 434.14 mg/mL,syringin was 86.37μg/mL,lobetyolin was 287.94μg/mL,and the extract was 65.11%.The process had the advantages of high extraction rate,simple operation,simple and economical reagents.