抗抑郁药蒲郁胶囊对雄性大鼠性功能的影响及其机制初探

Effect of antidepressant PuYu Capsule on sexual function of male rats and its mechanism

目的观察蒲郁胶囊对雄性大鼠性功能与血清雄激素的影响,并与米氮平和药氟西汀进行比较,研究蒲郁胶囊的药理学特点。方法60只雄性大鼠随机分为空白、氟西汀、米氮平和蒲郁胶囊低、中、高剂量共6组。重复给药1周和2周后,通过大鼠交配行为模型和非接触行为模型观察大鼠性功能的差异;之后分别加入五羟色胺1A受体拮抗剂WAY100635和部分激动剂丁螺环酮观察交配行为和非接触性阴茎勃起行为;最后取血,分离血清,ELISA法检测各组雄性大鼠血清雄激素水平。结果氟西汀对性功能有抑制作用,而蒲郁胶囊和米氮平对大鼠性功能均无影响;3种药物对大鼠雄激素均无影响。加入WAY100635和丁螺环酮后能逆转氟西汀引起的性功能障碍,对蒲郁胶囊无影响。结论蒲郁胶囊对雄性大鼠雄激素与性功能的影响均较小,优于氟西汀等SSRIs类抗抑郁药物,其原因可能与其不影响5-HT有关。

Aim To evaluate the effect of PuYu Cap-sule on sexual function in male rats in comparison to that of mirtazapine and SSRI antidepressant fluoxetine,so as to explore the pharmacological features of PuYu Capsule.Methods Sixty male rats were divided into control group,fluoxetine group,mirtazapine group,PuYu Capsule of high,medium and low dose group.After repeated administration of PuYu Capsule,the effects of the drugs on sexual function of male rats were verified by the mating behavior model and non-contact behavior model of rats.After repeated administration of PuYu Capsule,WAY100635 and buspirone were added respectively to verify the mating behavior model and non-contact behavior model.Furthermore,the effects of PuYu Capsule on rat androgen were verified by rat androgen ELISA kit after chronic administration.Results In the mating behavior test and non-contact penile erection experiment of male rats,PuYu Capsule yielded no effect on the sexual function of male rats.WAY100635 and buspirone reversed the sexual dysfunction induced by fluoxetine,but had no effects on other medicines.None of the medications had any effect on androgen.Conclusions PuYu Capsule has no significant effect on androgen and sexual function of male rats,which is better than that of fluoxetine.The antidepressant mechanism of PuYu Capsule may be that it does not affect 5-HT.