净作和套作下大豆贮藏蛋白11S、7S组分相对含量的QTL分析

QTL analysis for relative contents of glycinin and β-conglycinin fractions from storage protein in soybean seeds under monoculture and relay intercropping

大豆贮藏蛋白的7S组分和11S组分占蛋白质总量的70%左右,其含量直接与大豆种子的加工品质、营养品质及功能特性密切相关。目前,在玉米-大豆带状套作模式下11S和7S组分的相对含量会如何变化尚不明确。本研究以南豆12和九月黄为亲本所构建的重组自交系(RILs)群体为材料,结合已构建的SNP高密度遗传图谱,在不同环境(E1:2017年仁寿;E2:2017年雅安;E3:2016年仁寿)的净作及套作下,对控制贮藏蛋白组分含量的相关性状进行QTL分析。结果表明,在不同的环境和种植方式下,亲本和RIL群体的大部分贮藏蛋白11S、7S组分相对含量差异显著或极显著;检测到10个相关QTL,分布于9条染色体,表型贡献率为5.63%~9.68%。通过注释信息,在定位到的QTL区段中初步筛选出65个与贮藏蛋白质含量相关的候选基因。上述结果为大豆品质育种提供了理论参考。

The relative contents of glycinin andβ-conglycinin from storage protein are closely related to the quality and function of soybean seeds.However,it is not clear whether or how glycinin andβ-conglycinin contents change in maize-soybean strip intercropping system.The glycinin andβ-conglycinin relative contents of the recombinant inbred lines(RILs)derived from the cross of‘Nandou 12’and‘Jiuyuehuang’were measured under different environments(E1:2017,Renshou;E2:2017,Ya’an;E3:2016,Renshou)and planting patterns(M:monoculture;RI:relay intercropping),among which the differences were significant or extremely significant in parents and RILs.Based on a genetic linkage map with 6366 SNP markers,we detected ten QTLs for glycinin andβ-conglycinin relative contents which were distributed in nine linkage groups with the phenotypic variation of 5.63%–9.68%.According to the soybean genomic information,65 candidate genes were screened in the region of above-mentioned QTLs.These results lay a theoretical foundation for soybean quality breeding.