肝癌组织RhoA表达及与浸润转移的关系

Rho A expression in hepatic carcinoma tissues and relationship with hepatic carcinoma invasion and metastasis

目的检测RhoA在不同侵袭程度的肝癌组织中的表达,探讨其与肝癌浸润转移的关系。方法选取有完整临床资料、不同浸润程度及不同病理分期的52例肝癌组织及癌旁正常组织标本,应用荧光定量PCR法(QRT-PCR)及蛋白免疫印记法(Weastern blotting)检测RhoA表达情况。结果肝癌组织中RhoA mRNA及RhoA蛋白的表达量明显高于癌旁组织,并与肿瘤病理分期及侵袭程度密切相关,在门静脉受侵犯、有微卫星病灶和pTNM stage分期较晚的患者中明显增高。结论RhoA的表达与肝癌的浸润转移有关,可作为重要的生物学标志。

Objective To detect the expression of Rho A in different invasion degrees of hepatic carcinoma(HCC)tissues,and to explore the relationship between Rho A and the invasion and metastasis of liver cancer.Methods We selected 52 cases of liver cancer tissue and adjacent normal tissue samples,which have complete clinical data,the different degree of infiltration and pathological stage.Rho A expression was detected by quantitative PCR(QRT-PCR)and protein immunoblotting(Weastern blotting).Results Rho A mRNA and Rho A protein expression in HCC tissues were significantly higher than the adjacent tissues,and closely related to pathological stage and tumor invasion degree.It was significantly higher in patients with violations of the portal vein,microsatellite lesions and p TNM stage stage late.Conclusion Rho A expression is contact with the liver metastasis,and can be used as an important biological markers.