醒脑再造胶囊对甲醛致小鼠呼吸系统损害的影响

Effects of Xingnaozaizao capsules on formaldehyde-induced impairment of respiratory system in mice

目的观察醒脑再造胶囊对甲醛所致小鼠呼吸系统损害的影响。方法将小鼠随机分为5组:对照组(C组),甲醛组(FA组),低、中、高剂量醒脑再造胶囊组(XL组、XM组、XH组),每组10只。自实验第1天至第14天,除C组外,其余小鼠每天均进行甲醛熏蒸2 h,其中XL、XM和XH组每天还需分别用1.0、1.5、2.0 g·kg^-1的醒脑再造胶囊混悬液灌胃。实验第14天用酚红排泌试验评估支气管排泌功能,检测肺系数,用生化方法测定肺组织匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)的活性。结果与C组比较,FA组的酚红排泌量减少(P<0.05);肺组织匀浆中MDA含量增高(P<0.05),SOD和NOS活性均明显下降(P<0.01)。与FA组比较,XH组的酚红排泌量明显增多(P<0.01),肺组织匀浆中MDA含量明显降低(P<0.01),SOD和NOS活力均明显升高(P<0.01);XM组NOS活力升高(P<0.05)。结论醒脑再造胶囊可改善甲醛所致小鼠呼吸系统损害。

Objective To observe the effects of Xingnaozaizao capsules on formaldehyde-induced impairment of respiratory system in mice.MethodsKunming mice were randomly divided into 5 groups(n=10):a control group(Group C),a formaldehyde group(Group FA)and a low,middle and high dose of Xingnaozaizao capsule group(Groups XL,XM and XH).From Day 1 to Day 14 of the experiments,all mice except for Group C were exposed to formaldehyde for 2 h every day.Furthermore,Groups XL,XM and XH were orally administrated with Xingnaozaizao capsule suspension at a dose of 1.0,1.5 and 2.0 g·kg^-1.On Day 14,phenol red excretion test was used to evaluate the excretion function of the bronchi,and the lung coefficient of mice was tested.The contents of malonaldehyde(MDA)and the activities of superoxide dismutase(SOD)and nitricoxide synthase(NOS)in the lung homogenate were measured biochemically.ResultsCompared with Group C,Group FA demonstrated significantly decreased phenol red excretion(P<0.05),increased MDA contents in the lung homogenate(P<0.05)and reduced activities of SOD and NOS(P<0.01).Compared with Group FA,Group XH demonstrated significantly increased phenol red excretion(P<0.01),reduced MDA contents in the lung homogenate(P<0.01)and increased activities of SOD and NOS(P<0.01),while Group XM presented enhanced NOS activities(P<0.01).ConclusionsXingnaozaizao capsules can improve formaldehyde-induced impairment of respiratory system in mice.