超高效液相色谱-串联质谱法测定复合调味料中爱德万甜的不确定度评定

Uncertainty Evaluation of the Determination of Advantame in Compound Seasoning by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

采用超高效液相色谱-串联质谱法测定复合调味料中的爱德万甜,并建立不确定度评定模型。通过对不确定来源的分析计算,得出合成不确定度及扩展不确定度。根据结果表明,最小二乘拟合引入的不确定度影响最大。当取样量为1.0000 g,k=2(95%置信水平)时,测得爱德万甜理论含量为50μg/kg的复合调味料中爱德万甜的值为(50.03±2.98)μg/kg。

The advantame content in compound seasoning was determined by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),and the uncertainty evaluating model was estab-lished.Through the analysis of each component uncertainty,the combined standard uncertainty and the expand uncertainty were calculated.Comparing each component of uncertainty in the process,the results showed that the effect of the squares fitting was the maximum.The amount of advantame in compound seasoning with a theoretical content of advantame of 50μg/kg was(50.03±2.98)μg/kg when the sample weight was 1.0000 g,k=2(95%confidence).