2017—2018年云南省普洱市猪伪狂犬病野毒感染血清学调查

Serological Investigation on Infection with Wild PRV in Pu’er City of Yunnan Province during 2017 to 2018

为了解当前云南省普洱市猪伪狂犬病野毒感染情况,2017-2018年在普洱市10个县(区)的490户散养户和71个规模场,采集2035份猪血清样品,采用gE-ELISA方法进行猪伪狂犬病病毒gE抗体检测。结果显示:普洱市猪伪狂犬病病毒gE抗体平均阳性率为1.92%,其中规模场为2.26%,散养户为1.77%,种猪群为3.41%。数据统计分析发现:规模场和散养户的伪狂犬病野毒感染率差异不显著(P>0.05),而冬春季节与其他季节、10月龄以上与以下的感染率差异均显著(P<0.05)。综上,目前普洱市猪伪狂犬病野毒感染程度较轻,感染范围较小,但仍需加强防控,尤其是冬春季节和种猪群。本研究基本摸清了普洱市猪伪狂犬病的流行状况、流行范围以及高发季节和高风险猪群,从而为当地猪伪狂犬病净化策略的制定和实施提供了依据。

In order to recognize the infection status of wild porcine pseudorabies virus(PRV)in Pu’er City of Yunnan Province,2035 swine serum samples were collected from 490 free-range households and 71 large-scale farms in 10 counties(districts)from 2017 to 2018,and gE antibodies against PRV were detected by gE-ELISA.The results showed that the average positive rate of gE antibody was 1.92%,the rate was 2.26%for large-scale farms,1.77%for free-range households,and 3.41%for breeding herds.According to the analysis of statistical data,the difference of infection rates between large-scale farms and free-range households was not significant(P>0.05),while an obvious difference was observed in the infection rates between winter-spring season and other seasons,and between different-age pigs(more than 10 months or not)(P<0.05).In conclusion,the wild PRV was prevalent to a lesser extent in Pu’er City,with a small infection range,but it was necessary to intensively implement prevention and control measures,especially for breeding herds and in the seasons(winter and spring)with high incidence.In this study,the prevalence and spreading range of wild PRV,as well as the seasons with high incidence and pig herds at high risk were basically identified,which provided some references for formulating and implementing related purification strategies in the city.