过表达miR-140-5p促进前列腺癌细胞凋亡抑制其增殖

Overexpression of miR-140-5p promotes apoptosis and inhibits proliferation of prostate cancer cells

目的研究miR-140-5p在前列腺癌细胞中的表达情况,探讨miR-140-5p在前列腺癌细胞中的生物学功能。方法RT-qPCR方法检测前列腺癌细胞株PC3细胞及DU145细胞中miR-140-5p表达;PC3细胞中转染miR-140-5p模拟物或抑制物,CCK-8及AnnexinV/PI双染流式细胞学检测细胞活力及细胞凋亡。TCGA数据库分析高表达miR-140-5p与前列腺癌患者总生存率的关系。结果miR-140-5p在DU145及PC3细胞中的表达明显低于RWPE-1细胞,miR-140-5p在PC3细胞中的表达明显低于DU145细胞(P<0.05)。转染miR-140-5p模拟物的PC3细胞中miR-140-5p相对表达量明显高于阴性对照组(P<0.05)。miR-140-5p的模拟物转染PC3细胞48 h,过表达组细胞增殖率低于阴性对照组,凋亡率高于阴性对照组(P<0.05)。转染miR-140-5p抑制物的PC3细胞中miR-140-5p相对表达量明显低于阴性对照组(P<0.05)。敲低miR-140-5p表达组细胞增殖率明显高于阴性对照组,凋亡率明显低于阴性对照组(P<0.05)。低表达miR-140-5p组患者总生存率低于高表达miR-140-5p组患者(P<0.05)。结论miR-140-5p在前列腺癌细胞株低表达,低表达miR-140-5p前列腺癌患者预后不良;过表达miR-140-5p可促进前列腺癌细胞凋亡,抑制其增殖。

Objective To investigate the expression of miR-140-5p in prostate cancer cells and explore the biological functions of miR-140-5p in prostate cancer progression.Methods RT-qPCR was performed to identify the expression of miR-140-5p in PC3 and DU145 cell lines.CCK8 assay and AnnexinV-FITC/PI stain were used to exam cell proliferation cell apoptosis.TCGA database was used to analyze the relationship between miR-140-5p expression level and survival of prostate cancer patient.Results miR-140-5p was downregulated in PC3 and DU145 cells.Transfection of miR-140-5p mimic to PC3 cells significantly increased miR-140-5p level in PC3 cells.Overexpression of miR-140-5p in PC3 cells increased the cell apoptosis and decreased cell viability.Transfection of miR-140-5p inhibitor to PC3 cells significantly decreased miR-140-5p level in PC3 cells.Knockdown of miR-140-5p in PC3 cells decreased cell apoptosis and increased cell viability.Lower miR-140-5p level of prostate cancer patients had a poor overall survival analyzing from TCGA database.Conclusion miR-140-5p is downregulated in prostate cell lines and correlates with poor survival of prostate cancer.Overexpression of miR-140-5p in PC3 cells promotes cell apoptosis and inhibits cell growth.