TETs蛋白在乌拉坦诱导小鼠肺癌模型中的表达模式

Analysis of the expression pattern of TET proteins in lung cancer mouse model induced by urethane

目的探讨TET家族蛋白在小鼠肺癌模型中的表达模式。方法将小鼠分成3组,每组10只。分别设为对照组(Con),乌拉坦诱导组(Ure),以及羟基脲给药组(Ure-HU)。对Ure组和Ure-HU组连续10周给予800 mg/kg乌拉坦溶液,诱导建立小鼠肺癌摸型。10周后对Ure-HU组给予HU药物(500 mg/kg),连续21 d。观察肺部形态结节个数。利用q PCR和Western blot分析TETs在3组小鼠中的表达模式。结果与Con组相比,Ure和Ure-HU组体重下降明显。形态学观察显示,Ure组和Ure-HU组均有肺组织病变。q PCR结果显示,在Ure组,TETs表达显著降低(P<0.05)。经过HU处理后,Ure-HU组TET1和TET2表达上升(P<0.05)。Western blot与q PCR结果一致。结论在乌拉坦诱导的小鼠肺癌模型中,TETs蛋白表达模式异常,证明在肺癌发生过程中,TET1和TET2具有重要作用。

Objective The expression pattern of TET family proteins was investigated in a mouse lung cancer model.Methods The mice were divided into three groups(with 10 mice in each group):control group(Con),urethaneinduced group(Ure),and HU-treated group(Ure-HU).Urethane(800 mg/kg)was injected intraperitoneally for 10 weeks to establish the mouse lung cancer model in the Ure and Ure-HU groups.After 10 weeks,the Ure-HU group was given the drug HU(500 mg/kg)for 21 days.The morphology was observed thereafter,while q PCR and western blotting were used to analyze the expression patterns of TET1,TET2,and TET3 in the three groups.Results Compared with that of the Con group,the weights of the Ure group and Ure-HU group decreased.Morphological observation revealed lung tissue lesions in the Ure and Ure-HU groups.q PCR result suggested that the expression of TETs was decreased in the Ure group(P<0.05).After HU treatment,the expression of TET1 and TET2 was increased in the Ure-HU group(P<0.05).The data from western blotting were consistent with the q PCR result.Conclusions The expression of TETs was abnormal in a mouse lung cancer model induced by urethane.These result suggest that TET1 and TET2 play important roles in lung cancer.