大鼠心肌缺血再灌注损伤模型建立的改良方法

An improved method for establishing a rat model of myocardial ischemia-reperfusion injury

目的综合评估大鼠心肌缺血再灌注损伤(myocardial ischemia-reperfusion injury,MIRI)模型建立的改良方法。方法将45只SD大鼠随机分为3组:快速操作组、常规开胸组和改良组,每组15只。分别采用快速操作、常规开胸造模及改良方法进行大鼠心肌缺血再灌注手术造模,收集大鼠心脏进行TTC+伊文思蓝染色观察,分别从造模成功率、大鼠存活率、心肌损伤情况三方面进行分析比较。结果与快速造模和开胸造模组相比,改良方法造模的成功率、成活率均有所提高,且心肌梗死面积厚度统一。结论心肌缺血再灌注损伤模型构建方法改良后的可行性较高,可推广应用。

Objective Comprehensive evaluation of an improved method of inducing myocardial ischemiareperfusion injury(MIRI)in rats.Methods Forty-five SD rats were randomly divided into three groups:fast operation group,conventional thoracotomy group,and improved group(n=15 in each group).Rat models of MIRI were established by rapid operation,routine thoracotomy,and the improved method,respectively.TTC+Evans blue staining was used to observe rat hearts.The degree of injury was analyzed and compared regarding three aspects:success rate of modeling,survival rate of rats,and myocardial injury.Results Compared with the fast operation group and conventional thoracotomy group,the success rate and survival rate with the improved method were improved,and the area and thickness of myocardial infarction were the same.Conclusions The improved method of MIRI modeling is feasible and can be popularized.