当归藤补血有效部位的筛选及其补血机制研究

Study on Screening of Effective Components of Embelia parviflora for Tonifying Blood and Its Mechanism

目的:考察当归藤不同提取部位对血虚模型小鼠的补血作用,并探索其作用机制。方法:将70只小鼠随机分为空白对照组(水)、模型对照组(水)、阳性对照组(当归补血口服液,324 g/kg)和当归藤石油醚部位、乙酸乙酯部位、正丁醇部位、水部位组(给药剂量依次为4.2、10.64、22.07、5.0 g/kg,以提取部位浸膏计),每组10只。小鼠均灌胃给药,每天1次,连续给药14 d。除空白对照组外,其余各组小鼠均于开始给药的第12、13天时腹腔注射环磷酰胺(80 mg/kg)复制血虚模型。末次给药30 min后,采用全自动血细胞分析仪检测小鼠外周血象指标[白细胞计数(WBC)、红细胞计数(RBC)、红细胞压积(HCT)、血小板计数(PLT)和血红蛋白浓度(HGB)]水平;采用酶联免疫吸附法测定小鼠血清中白细胞介素2(IL-2)、IL-3、IL-6、促红细胞生成素(EPO)、粒细胞集落刺激因子(G-CSF)、巨噬细胞集落刺激因子(M-CSF)、血管细胞黏附分子1(VCAM-1)水平;计算小鼠胸腺指数与脾指数。结果:与空白对照组比较,模型对照组小鼠各血象指标水平、血清中IL-2、IL-3、IL-6、EPO、G-CSF、M-CSF、VCAM-1水平以及胸腺指数均显著降低,脾指数显著升高(P<0.01)。与模型对照组比较,当归藤石油醚部位组小鼠上述指标差异均无统计学意义(P>0.05);当归藤乙酸乙酯部位组小鼠外周血RBC、HGB、PLT水平、血清中IL-2、IL-6、G-CSF、M-CSF、VCAM-1水平以及胸腺指数显著升高(P<0.05或P<0.01),其余指标差异无统计学意义(P>0.05);除当归藤水部位组小鼠WBC升高不显著外,当归藤正丁醇部位、水部位组小鼠上述各血象指标和血清因子水平及胸腺指数均显著升高,脾指数均显著降低(P<0.05或P<0.01)。结论:当归藤乙酸乙酯、正丁醇和水部位均可提高血虚模型小鼠的免疫功能和造血因子的表达,具有一定的补血作用。

OBJECTIVE:To investigate the tonifying blood effects of different extract parts of Embelia parviflora on blood deficiency model mice,and to explore its mechanism.METHODS:Totally 70 mice were randomly divided into blank control group(water),model control group(water),positive control group(Danggui buxue oral liquid,324 g/kg),petroleum ether,ethyl acetate,n-butanol and water parts of E.parviflora groups(4.2,10.64,22.07,5.0 g/kg respectively,calculated by the extractum),with 10 mice in each group.The mice were given medicine intragastrically,once a day,for consecutive 14 d.Except for blank control group,other groups were given intraperitoneal injection of cyclophosphamide(80 mg/kg)on 12th and 13th day of starting administration to induce blood deficiency model.30 min after last administration,automatic hematology analyzer was used to detect the levels of peripheral hemogram indexes(WBC,RBC,HCT,PLT,HGB);the levels of IL-2,IL-3,IL-6,EPO,G-CSF,M-CSF and VCAM-1 were determined by ELISA;thymus and spleen indexes were calculated.RESULTS:Compared with blank control group,peripheral hemogram indexes levels,serum levels of IL-2,IL-3,IL-6,EPO,G-CSF,M-CSF,VCAM-1 and thymus index were decreased significantly,while spleen index was increased significantly(P<0.01).Compared with model control group,there was no statistical significance in above indexes of mice in petroleum ether part of E.parviflora group(P>0.05).The levels of RBC,HGB,PLT,the serum levels of IL-2,IL-6,G-CSF,M-CSF,VCAM-1 and thymus index in ethyl acetate part of E.parviflora group were significantly increased(P<0.05 or P<0.01),while there was no statistical significance in other indexes(P>0.05).Except for no significant increase of WBC in water part of E.parviflora group,above peripheral hemogram indexes,serum indexes and thymus index of n-butanol group and water part of E.parviflora group were increased significantly while spleen index was decreased significantly(P<0.05 or P<0.01).CONCLUSIONS:The ethyl acetate,n-butanol and water parts of E.parviflora can improve immunological function and the expression of hematopoietic factors in blood deficiency model mice,and shows certain blood tonifying effects.