BRAF V600E基因检测联合细针穿刺活检对甲状腺结节良恶性诊断中的价值

Diagnostic value of BRAF V600E gene mutation detection combined with fine needle aspiration biopsies in benign and malignant thyroid nodules

目的:探讨BRAF V600E基因检测联合超声引导下细针穿刺活检(fine needle aspiration biopsy,FNA)对甲状腺良恶性结节的临床诊断价值。方法:选取2018年1月至2018年6月首次收入郑州大学附属肿瘤医院穿刺活检并手术的121例患者共130枚甲状腺结节,对穿刺标本行细胞学诊断及洗脱液BRAF V600E突变基因检测。结果:130枚结节中术后病理诊断乳头状癌120枚,良性10枚,BRAF V600E基因突变检测联合FNA将细胞学诊断的敏感度由81.7%提升至92.5%,准确度由83.1%升至92.3%,差异有统计学意义(P<0.05)。结论:BRAF V600E基因检测联合FNA能有效提高甲状腺结节良恶性诊断的敏感度和准确度,比传统单纯细胞学诊断具有更大的参考价值。

Objective: To detect and evaluate the importance of the BRAF V600E gene mutation in ultrasound-guided fine needle aspiration biopsies(FNA) for the diagnosis of benign and malignant thyroid nodules. Method: Totally, 121 patients who were admitted to the hospital for the first time between January 2018 and June 2018 were included in this study. Cytological diagnosis and evaluation of 130 thyroid nodules from these patients were performed for detecting the BRAF V600E gene mutation. Results: Among the 130 nodules screened, 120 were postoperative pathological papillary carcinoma and 10 were benign. The analysis of the BRAF V600E gene mutation combined with FNA increased the sensitivity of cytological diagnosis from 81.7% to 92.5%(P<0.05). The difference was statistically significant. Conclusions: BRAF V600E gene mutation detection combined with FNA can effectively improve the sensitivity and accuracy of diagnosis of benign and malignant thyroid nodules and has greater potential than traditional and simple cytological diagnostic methods.