MiR-92a表达上调与结直肠癌临床病理及预后的相关性分析

Correlation between up-regulation of miR-92a expression and clinical pathology and prognosis of colorectal cancer

目的分析miR-92a表达与结直肠癌临床病理特征和预后的相关性。方法应用原位杂交方法,检测miR-92a在124例结直肠癌组织中的表达,取中位表达水平作为阈值将患者分为miR-92a高表达组和miR-92a低表达组。利用卡方检验及Spearman相关检验,分析miR-92a表达与结直肠癌临床病理特点的相关性。应用Kaplan-Meier方法分析结直肠癌患者术后3年生存率及生存时间。利用单因素及多因素Cox回归模型分析结直肠癌患者预后的独立危险因素。结果MiR-92a表达与结直肠癌分化程度(P=0.015)、浸润深度(P=0.031)、淋巴结转移(P=0.030)、远处转移(P=0.011)和AJCC临床分期(P=0.047)存在显著相关性。Kaplan-Meier生存分析显示高表达和低表达miR-92a组患者术后3年累积生存率分别为66.1%和91.9%(χ^2=12.006,P=0.001),中位生存时间分别为29.350(95%CI:26.627~32.074)个月和34.939(95%CI:33.078~36.799)个月。多因素Cox分析显示,miR-92a表达是一个独立的结直肠癌预后评估因素(P=0.026)。结论结直肠癌组织中miR-92a表达上调与肿瘤分化程度、浸润深度、淋巴结转移、远处转移和AJCC临床分期存在显著相关性;miR-92a表达可作为预测结直肠癌患者远期预后的分子标志物。

Objective To analyze the correlation between the expression of microRNA-92a(miR-92a)and clinicopathological characteristics in tissue of colorectal cancer.Methods In situ hybridization was used to detect the expression of miR-92a in 124 colorectal cancer tissues.The median expression level was used as the threshold to divide patients into miR-92a high expression group and miR-92a low expression group.Statistical analysis of chi-square test,Kaplan-Meier analysis,univariate and multivariate Cox regression models were used to determine the potential correlation between miR-92a expression and clinicopathological features or prognosis of colorectal cancer.Results The expression miR-92a was significantly correlated with the degree of differentiation(P=0.015),depth of invasion(P=0.031),lymph node metastasis(P=0.030),distant metastasis(P=0.011)and AJCC clinical stage(P=0.047).Kaplan-Meier survival analysis showed that the 3-year cumulative survival rates were 66.1%and 91.9%(χ^2=12.006,P=0.001)in patients with high and low expression of miR-92a respectively.Multivariate Cox analysis showed that the expression of miR-92a was an independent prognostic factor for colorectal cancer(P=0.026).Conclusion The expression of miR-92a is significantly correlated with the clinical stage of colorectal cancer and can be used as a molecular marker to predict the long-term prognosis of colorectal cancer.