贝赫切特综合征患者肠道菌群微生物宏基因组学分析

Metagenomics analysis of intestinal flora microbes in patients with Behcet Syndrome

目的采用宏基因组学分析贝赫切特综合征(BD)患者肠道菌群微生物特征。方法选取2016年1月至2018年1月的BD患者18例,另选取性别、年龄、BMI相匹配的健康人群24例作为对照,收集BD患者与健康人群新鲜粪便提取DNA,进行宏基因组测序及生物信息学分析。结果BD患者与正常人优势菌群主要是硬壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和变形菌门(Proteobacteria)。BD患者中Bacteroidetes占4.21%,远低于正常人,BD患者中Proteobacteria占42.80%,远高于正常人;BD患者与正常人胃肠道微生物在属水平上有194个菌属存在差异,在种水平上有887个菌种存在差异;BD患者优势菌包括Collinsella Spp.、Bilophila spp.及Bacteroides spp.等,正常人优势菌包括Clostridium spp.Roseburia spp.Methanogens等。结论BD患者肠道菌群微生物群发生改变,产乳酸菌Collinsella Spp.和硫酸盐还原菌Bilophila spp.增多,产丁酸菌Clostridium spp.和Roseburia spp.减少,可能为引起BD发病的机制之一。

Objective To analyze the microbial characteristics of intestinal flora in patients with Behcet syndrome(BD)analyzed by metagenomics.Methods Eighteen BD patients from January 2016 to January 2018 were selected,and another 24 healthy people matched with gender,age,and BMI were selected as controls.DNA was extracted from fresh feces of BD patients and healthy people for metagenome sequencing and bioinformatics analysis.Results The dominant floras in BD patients and normal people were mainly Firmicutes,Bacteroidetes and Proteobacteria.The proportion of Bacteroidetes with 4.21%in BD patients was much lower than that inhealthy controls while the proportions of Proteobacteria with 42.80%in BD patients was much higher than that in controls.There were differences between BD patients and controls in the 194 genera at the genus level and 887 strains at species level of gastrointestinal microbes.The dominant floras in BD patients included Collinsella Spp.,Bilophila spp.and Bacteroides spp.,etc.The dominant floras in healthy controls included Clostridium spp.,Roseburia spp.,Methanogens.Conclusion The microbial population of intestinal flora is changed in BD patients.The increases of the lactic acid⁃producing bacteria Collinsella Spp.and the sulfate⁃reducing bacteria Bilophila spp.and the decreases of the butyric acid⁃producing bacteria Clostridium spp.And Roseburia spp.may be one of the mechanisms of causing BD.