HPLC同时测定肺宁制剂中4种成分的含量

Simultaneous Determination of Four Constituents in Feining Preparation by HPLC

目的建立HPLC同时测定肺宁颗粒、肺宁胶囊和肺宁片中新绿原酸、绿原酸、隐绿原酸和金丝桃苷4种成分的含量。方法采用Waters XBridge TM C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%磷酸水溶液,梯度洗脱,流速1.0 mL·min^-1;检测波长327,353 nm;柱温30℃。结果4种成分在各自范围内线性关系良好(r>0.9990),平均加样回收率96.1%~104.7%,RSD为0.4%~1.6%。结论该方法准确、可靠,重复性好,可用于肺宁制剂的质量控制。

OBJECTIVE To establish an HPLC method for the simultaneous determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid and hyperin in Feining granules,Feining capsules and Feining tablets.METHODS Preparation was performed on a 30℃Waters XBridge TM C18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile-0.4%phosphoric acid flowing at 1.0 m L·min^-1 in a gradient elution manner,and the detection wavelengths were set at 327,353 nm.RESULTS Four constituents showed good linear relationships with their own ranges(r>0.9990),while the average recoveries were 96.1%-104.7%with the RSDs of 0.4%-1.6%.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Feining preparation.