当归对阴虚哮喘小鼠气道杯状细胞及JAK1/STAT6信号通路的影响

Effects of Angelicae Sinensis Radix on Airway Goblet Cells and JAK1/STAT6 Signaling Pathway in Asthmatic Mice with Yin Deficiency Syndrome

目的探讨当归对阴虚哮喘小鼠气道杯状细胞增生及JAK1/STAT6信号通路的影响。方法采用卵清白蛋白与甲状腺复制阴虚哮喘动物模型,观测当归对阴虚哮喘小鼠肺通气功能、哮喘气道杯状细胞增生、肺组织中Muc5ac、IL-13、IL-4含量以及JAK1与STAT6表达水平的影响。结果当归可明显缓解阴虚哮喘小鼠呼吸频率、潮气量及增强呼气间歇的异常变化,改善肺功能,降低肺指数及肺组织湿干重比值,抑制气道杯状细胞增生,降低肺组织中Muc5ac、IL-13及IL-4含量,抑制肺组织JAK1及STAT6蛋白的表达(P<0.05或P<0.01)。结论当归具有明显缓解气道杯状细胞增生及Muc5ac高表达而平喘的作用,抑制JAK1/STAT6信号通路是其改善哮喘气道黏液分泌失衡的作用机制之一。

OBJECTIVE To investigate the effect of Angelicae Sinensis Radix on airway goblet cell hyperplasia and JAK1/STAT6 signaling pathway in asthmatic mice with Yin deficiency syndrome.METHODS The asthmatic with Yin deficiency syndrome model was established with ovalbumin and thyroxin,then to observe the effects of Angelicae Sinensis Radix on pulmonary ventilation function,asthmatic airway goblet cell hyperplasia,Muc5 ac,IL-13 and IL-4 contents in lung tissues,JAK1 and STAT6 expression levels in lung tissues of asthmatic mice with Yin deficiency.RESULTS Angelicae Sinensis Radix could significantly alleviate the abnormal changes of respiratory rate,tidal volume and enhance pause in asthmatic mice with Yin deficiency syndrome,improve lung function,reduce pulmonary index and wet to dry weight ratio of lung tissue,restrain airway goblet cell hyperplasia,decrease levels of Muc5 ac,IL-13 and IL-4 in lung tissue,restrain JAK1 and STAT6 protein expression in lung tissue(P<0.05 or P<0.01).CONCLUSION Angelicae Sinensis Radix can significantly alleviate airway goblet cell hyperplasia and decrease the high expression of Muc5 ac and relieve asthma.One of the mechanisms to improve the imbalance of airway mucus secretion in asthma is the inhibition of JAK1/STAT6 signaling pathway.