一测多评法比较不同产地甘草中6种有效成分含量

Simultaneous Quantitative Analysis of Six Active Ingredients in Glycyrrhizae Radix Et Rhizoma from Different Habitats by QAMS

目的建立甘草中6种成分含量的一测多评方法,并验证该方法的准确性和可行性。方法以甘草苷为内参物,分别建立甘草酸、芹糖甘草苷、芹糖异甘草苷、甘草素、异甘草素的相对校准因子,利用该相对校准因子计算其他5种成分含量,同时利用外标法测定不同产地和批次甘草中6种有效成分含量,比较2种测量方法的差异,验证一测多评法的可行性和准确性。结果16批不同产地和批号的甘草中6种有效成分采用一测多评法与外标法的实测值之间无显著性差异。结论一测多评法测定甘草中6种成分准确可行,可用于甘草的质量控制。

OBJECTIVE To establish quantitative analysis of multi-components to a single-marker(QAMS) for determining contents of six ingredients from Glycyrrhizae Radix et Rhizoma and to verify the accuracy and feasibility of the method. METHODS QAMS was established and validated for Glycyrrhizae Radix et Rhizoma. Liquiritin apioside, isoliquiritin apioside, liquiritiyenin, isoliquiritiyenin and glycyrrhiic acid were selected an analytes while liquiritin was chosen as internal reference substance to evalyaet the quality. The relative correction factor(RCF) of liquiritin to the other five active ingredients were calculated. The method was evaluated by the comparison of quantity between external standard method and QAMS method. RESULTS The contents of six active ingredients in 16 batches of Glycyrrhizae Radix et Rhizoma determined by QAMS method had no significantly different with external standard method. CONCLUSION The determination of 6 components in Glycyrrhizae Radix et Rhizoma by QAMS is accurate and feasible, which can be used for the quality control of Glycyrrhizae Radix et Rhizoma.