摘要
DNA组装是合成生物学研究的核心技术。随着合成生物学的发展,研究者开发了依赖于DNA聚合酶或DNA连接酶的不同DNA组装技术;为了降低组装成本和便于实现DNA组装的自动化,也发展了一些非酶依赖的DNA组装技术;而几百kb到Mb的大片段DNA的组装则多数依赖于微生物体内重组。文中主要综述了酶依赖、非酶依赖和体内同源重组三类DNA组装技术及其发展情况。
DNA assembly is the core technology of synthetic biology.With the development of synthetic biology,researchers have developed different DNA assembly technologies that rely on DNA polymerase or DNA ligase,and also have developed some non-enzyme-dependent DNA assembly techniques to facilitate the automation of DNA assembly.The assembly of large fragments of DNA from a few hundred kb to Mb is mostly dependent on microbial recombination.In this paper,the three types of DNA assembly technologies,including enzyme-dependent,non-enzymatic and in vivo homologous recombination,are reviewed.
作者
常汉臣
王琛
王培霞
周见庭
李炳志
Hanchen Chang;Chen Wang;Peixia Wang;Jianting Zhou;Bingzhi Li(Frontier Science Center for Synthetic Biology,Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300072,China;Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2019年第12期2215-2226,共12页
Chinese Journal of Biotechnology
基金
国家重点研发计划(No.2018YFA0900100)资助~~
关键词
合成生物学
DNA组装
酶依赖
同源重组
合成
synthetic biology
DNA assembly
enzyme-dependent
homologous recombination
synthesis
