美洲大蠊提取物对博来霉素诱导大鼠肺纤维化的疗效研究

Effect of Periplaneta Americana Extract on Bleomycin-induced Pulmonary Fibrosis in Rats

目的探讨美洲大蠊抗肺纤维化活性提取物(ML-HB)对博来霉素诱导肺纤维化大鼠的保护作用。方法将SD雄性大鼠随机分为正常组、模型组、阳性组(地塞米松3 mg·kg^-1,qd)及ML-HB高、中、低剂量组(120,60,30 mg·kg^-1,qd),采用气管暴露式注入博来霉素(5 mg·kg^-1)制备大鼠肺纤维化模型,造模24 h后经腹腔给药干预。给药第30天处死大鼠,测定肺指数,ELISA法检测肺匀浆中肿瘤坏死因子-α(tumornecrosisfactor-α,TNF-α)和转化生长因子-β1(transforming growthfactor-β1,TGF-β1)的表达,紫外法检测羟脯氨酸(hydroxyproline,HYP)的表达,HE染色观察肺组织炎症病变,Masson染色判定肺纤维化程度,免疫组化法观察α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)与I型胶原蛋白(type I collagen,COL-I)的表达。结果与正常组比,模型组肺指数和TNF-α、TGF-β1、HYP表达均升高(P<0.05或P<0.01),肺组织炎症和肺纤维化程度较严重、α-SMA与COL-I的表达均升高。ML-HB中剂量组较模型组肺指数和TNF-α、TGF-β1、HYP表达均降低(P<0.05或P<0.01),肺组织炎症和肺纤维化程度减轻、α-SMA与COL-I的表达均降低。结论 ML-HB可降低TNF-α、TGF-β1、HYP、α-SMA和COL-I的表达,延缓肺组织病变、干预纤维化的进一步发展。

OBJECTIVE To investigate the protective effect of anti-pulmonary fibrosis active extract from Periplaneta Americana(ML-HB) on bleomycin-induced pulmonary fibrosis in rats.METHODS Male SD rats were randomly divided into normal group,model group,positive group(dexamethasone,3 mg·kg^-1,qd) and ML-HB high,middle and low dosages groups(120,60,30 mg·kg^-1,qd).The pulmonary fibrosis model was established through tracheotomy with bleomycin(5 mg·kg^-1).The rats were intervened by treating with intraperitoneal administration after modeling 24 h.The rats were sacrificed on the 30 th day after administration,lung index was determined,tumor necrosis factor-α(TNF-α) and transforming growth factor-β1(TGF-β1) in lung homogenate were detected by ELISA,hydroxyproline(HYP) was detected by UV,the inflammatory lesions of lung tissue was observed by HE staining,the degree of pulmonary fibrosis was determined by Masson staining,and α-smooth muscle actin(α-SMA) and type I collagen(COL-I) were observed by immunohistochemical staining.RESULTS Compared with the normal group,the model group showed higher lung index,and higher expression of TNF-α,TGF-β1 and HYP(P<0.05 or P<0.01),lung tissue inflammation and pulmonary fibrosis were serious,α-SMA and COL-I expression were elevated.Compared with the model group,the ML-HB middle dosage group showed lower lung index,and lower expression of TNF-α,TGF-β1,and HYP(P<0.05 or P<0.01),lung tissue inflammation and pulmonary fibrosis were alleviated,α-SMA and COL-I expression were decreased.CONCLUSION ML-HB can reduce the expression of TNF-α,TGF-β1,HYP,α-SMA and COL-I,and delay lung tissue lesions,interfere with the further development of fibrosis.